8of7: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[8of7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NL15-2K Streptomyces sp. NL15-2K]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OF7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[8of7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._NL15-2K Streptomyces sp. NL15-2K]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OF7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8of7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8of7 OCA], [https://pdbe.org/8of7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8of7 RCSB], [https://www.ebi.ac.uk/pdbsum/8of7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8of7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8of7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8of7 OCA], [https://pdbe.org/8of7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8of7 RCSB], [https://www.ebi.ac.uk/pdbsum/8of7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8of7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/A0A401MXE6_9ACTN A0A401MXE6_9ACTN]  
[https://www.uniprot.org/uniprot/A0A401MXE6_9ACTN A0A401MXE6_9ACTN]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Stereoselective carbon-carbon bond forming reactions are quintessential transformations in organic synthesis. One example is the Diels-Alder reaction, a [4+2] cycloaddition between a conjugated diene and a dienophile to form cyclohexenes. The development of biocatalysts for this reaction is paramount for unlocking sustainable routes to a plethora of important molecules. To obtain a comprehensive understanding of naturally evolved [4+2] cyclases, and to identify hitherto uncharacterised biocatalysts for this reaction, we constructed a library comprising forty-five enzymes with reported or predicted [4+2] cycloaddition activity. Thirty-one library members were successfully produced in recombinant form. In vitro assays employing a synthetic substrate incorporating a diene and a dienophile revealed broad-ranging cycloaddition activity amongst these polypeptides. The hypothetical protein Cyc15 was found to catalyse an intramolecular cycloaddition to generate a novel spirotetronate. The crystal structure of this enzyme, along with docking studies, establishes the basis for stereoselectivity in Cyc15, as compared to other spirotetronate cyclases.
Interrogation of an Enzyme Library Reveals the Catalytic Plasticity of Naturally Evolved [4+2] Cyclases.,Zorn K, Back CR, Barringer R, Chadimova V, Manzo-Ruiz M, Mbatha SZ, Mobarec JC, Williams SE, van der Kamp MW, Race PR, Willis CL, Hayes MA Chembiochem. 2023 Jun 12:e202300382. doi: 10.1002/cbic.202300382. PMID:37305956<ref>PMID:37305956</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8of7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
</StructureSection>
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