1l2z: Difference between revisions
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New page: left|200px<br /> <applet load="1l2z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2z" /> '''CD2BP2-GYF domain in complex with proline-r... |
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== | ==CD2BP2-GYF domain in complex with proline-rich CD2 tail segment peptide== | ||
Intracellular protein interaction domains are essential for eukaryotic | <StructureSection load='1l2z' size='340' side='right'caption='[[1l2z]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1l2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2Z FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2z OCA], [https://pdbe.org/1l2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2z RCSB], [https://www.ebi.ac.uk/pdbsum/1l2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2z ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CD2B2_HUMAN CD2B2_HUMAN] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2z_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2z ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Intracellular protein interaction domains are essential for eukaryotic signaling. In T cells, the CD2BP2 adaptor binds two membrane-proximal proline-rich motifs in the CD2 cytoplasmic tail via its GYF domain, thereby regulating interleukin-2 production. Here we present the structure of the GYF domain in complex with a CD2 tail peptide. Unlike SH3 domains, which use two surface pockets to accommodate proline residues of ligands, the GYF domain employs phylogenetically conserved hydrophobic residues to create a single interaction surface. NMR analysis shows that the Fyn but not the Lck tyrosine kinase SH3 domain competes with CD2BP2 GYF-domain binding to the same CD2 proline-rich sequence in vitro. To test the in vivo significance of this competition, we used co-immunoprecipitation experiments and found that CD2BP2 is the ligand of the membrane-proximal proline-rich tandem repeat of CD2 in detergent-soluble membrane compartments, but is replaced by Fyn SH3 after CD2 is translocated into lipid rafts upon CD2 ectodomain clustering. This unveils the mechanism of a switch of CD2 function due to an extracellular mitogenic signal. | |||
Dynamic interaction of CD2 with the GYF and the SH3 domain of compartmentalized effector molecules.,Freund C, Kuhne R, Yang H, Park S, Reinherz EL, Wagner G EMBO J. 2002 Nov 15;21(22):5985-95. PMID:12426371<ref>PMID:12426371</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1l2z" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Freund | [[Category: Freund C]] | ||
[[Category: Kuhne | [[Category: Kuhne R]] | ||
[[Category: Park | [[Category: Park S]] | ||
[[Category: Reinherz | [[Category: Reinherz EL]] | ||
[[Category: Wagner | [[Category: Wagner G]] | ||
[[Category: Yang | [[Category: Yang H]] | ||
Latest revision as of 08:44, 22 May 2024
CD2BP2-GYF domain in complex with proline-rich CD2 tail segment peptide
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