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[[Image:1lf9.gif|left|200px]]
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{{STRUCTURE_1lf9|  PDB=1lf9  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE'''


==CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE==
<StructureSection load='1lf9' size='340' side='right'caption='[[1lf9]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lf9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LF9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900007:alpha-acarbose'>PRD_900007</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lf9 OCA], [https://pdbe.org/1lf9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lf9 RCSB], [https://www.ebi.ac.uk/pdbsum/1lf9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lf9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O85672_THETR O85672_THETR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lf/1lf9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lf9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain.


==Overview==
Crystal structure and evolution of a prokaryotic glucoamylase.,Aleshin AE, Feng PH, Honzatko RB, Reilly PJ J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:12614608<ref>PMID:12614608</ref>
The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1LF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF9 OCA].
</div>
<div class="pdbe-citations 1lf9" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure and evolution of a prokaryotic glucoamylase., Aleshin AE, Feng PH, Honzatko RB, Reilly PJ, J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12614608 12614608]
*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
[[Category: Glucan 1,4-alpha-glucosidase]]
*[[Amylase 3D structures|Amylase 3D structures]]
[[Category: Single protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermoanaerobacterium thermosaccharolyticum]]
[[Category: Thermoanaerobacterium thermosaccharolyticum]]
[[Category: Aleshin, A E.]]
[[Category: Aleshin AE]]
[[Category: Feng, P H.]]
[[Category: Feng P-H]]
[[Category: Honzatko, R B.]]
[[Category: Honzatko RB]]
[[Category: Reilly, P J.]]
[[Category: Reilly PJ]]
[[Category: 6 alpha-helical hairpin torroid]]
[[Category: Acarbose]]
[[Category: Carbohydrase family gh15]]
[[Category: Super beta sandwich]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:52:08 2008''
Retrieved from "https://proteopedia.org/1lf9"