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[[Image:1ltx.gif|left|200px]]
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{{STRUCTURE_1ltx|  PDB=1ltx  |  SCENE=  }}
'''Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid'''


==Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid==
<StructureSection load='1ltx' size='340' side='right'caption='[[1ltx]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ltx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LTX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAR:FARNESYL'>FAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ltx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ltx OCA], [https://pdbe.org/1ltx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ltx RCSB], [https://www.ebi.ac.uk/pdbsum/1ltx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ltx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGTA_RAT PGTA_RAT] Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/1ltx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ltx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies.


==Overview==
Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase.,Pylypenko O, Rak A, Reents R, Niculae A, Sidorovitch V, Cioaca MD, Bessolitsyna E, Thoma NH, Waldmann H, Schlichting I, Goody RS, Alexandrov K Mol Cell. 2003 Feb;11(2):483-94. PMID:12620235<ref>PMID:12620235</ref>
Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1LTX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA].
</div>
<div class="pdbe-citations 1ltx" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase., Pylypenko O, Rak A, Reents R, Niculae A, Sidorovitch V, Cioaca MD, Bessolitsyna E, Thoma NH, Waldmann H, Schlichting I, Goody RS, Alexandrov K, Mol Cell. 2003 Feb;11(2):483-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12620235 12620235]
*[[Geranylgeranyl transferase 3D structures|Geranylgeranyl transferase 3D structures]]
[[Category: Protein complex]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Alexandrov, K.]]
[[Category: Alexandrov K]]
[[Category: Goody, R S.]]
[[Category: Goody RS]]
[[Category: Niculae, A.]]
[[Category: Niculae A]]
[[Category: Pylypenko, O.]]
[[Category: Pylypenko O]]
[[Category: Rak, A.]]
[[Category: Rak A]]
[[Category: Reents, R.]]
[[Category: Reents R]]
[[Category: Schlichting, I.]]
[[Category: Schlichting I]]
[[Category: Thoma, N H.]]
[[Category: Thoma NH]]
[[Category: Waldmann, H.]]
[[Category: Waldmann H]]
[[Category: Lucine-rich repeat]]
[[Category: Post-translational modification]]
[[Category: Prenyltransferase]]
[[Category: Rab prenylation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:17:05 2008''

Latest revision as of 07:17, 25 October 2023

Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid

1ltx, resolution 2.70Å

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