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New page: left|200px<br /> <applet load="1lt8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lt8, resolution 2.05Å" /> '''Reduced Homo sapien...
 
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[[Image:1lt8.gif|left|200px]]<br />
<applet load="1lt8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lt8, resolution 2.05&Aring;" />
'''Reduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-Homocysteine'''<br />


==Overview==
==Reduced Homo sapiens Betaine-Homocysteine S-Methyltransferase in Complex with S-(delta-carboxybutyl)-L-Homocysteine==
Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of, methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to, activate Hcy. BHMT is a key liver enzyme that is important for, homocysteine homeostasis. X-ray structures of human BHMT in its oxidized, (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the, bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined, at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that, is distorted to construct the substrate and metal binding sites. The zinc, binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands, beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of, Zn are accompanied by local rearrangements. The structures identify Hcy, binding fingerprints and provide a prototype for the homocysteine, S-methyltransferase family.
<StructureSection load='1lt8' size='340' side='right'caption='[[1lt8]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lt8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LT8 FirstGlance]. <br>
1LT8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, CBH and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Betaine--homocysteine_S-methyltransferase Betaine--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.5 2.1.1.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LT8 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBH:S-(D-CARBOXYBUTYL)-L-HOMOCYSTEINE'>CBH</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lt8 OCA], [https://pdbe.org/1lt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lt8 RCSB], [https://www.ebi.ac.uk/pdbsum/1lt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lt8 ProSAT]</span></td></tr>
Betaine-homocysteine methyltransferase: zinc in a distorted barrel., Evans JC, Huddler DP, Jiracek J, Castro C, Millian NS, Garrow TA, Ludwig ML, Structure. 2002 Sep;10(9):1159-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12220488 12220488]
</table>
[[Category: Betaine--homocysteine S-methyltransferase]]
== Function ==
[https://www.uniprot.org/uniprot/BHMT1_HUMAN BHMT1_HUMAN] Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/1lt8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lt8 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Castro, C.]]
[[Category: Castro C]]
[[Category: Evans, J.C.]]
[[Category: Evans JC]]
[[Category: Garrow, T.A.]]
[[Category: Garrow TA]]
[[Category: Huddler, D.P.]]
[[Category: Huddler DP]]
[[Category: Jiracek, J.]]
[[Category: Jiracek J]]
[[Category: Ludwig, M.L.]]
[[Category: Ludwig ML]]
[[Category: Millian, N.S.]]
[[Category: Millian NS]]
[[Category: CBH]]
[[Category: CIT]]
[[Category: ZN]]
[[Category: homocysteine metabolism]]
[[Category: homocysteinemia]]
[[Category: thiol alkyl transfer]]
[[Category: transferase]]
[[Category: zinc]]
 
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