1ma8: Difference between revisions

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[[Image:1ma8.gif|left|200px]]


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==A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine==
The line below this paragraph, containing "STRUCTURE_1ma8", creates the "Structure Box" on the page.
<StructureSection load='1ma8' size='340' side='right'caption='[[1ma8]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MA8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr>
{{STRUCTURE_1ma8|  PDB=1ma8  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [https://pdbe.org/1ma8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ma8 ProSAT]</span></td></tr>
 
</table>
'''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Carrasco N]]
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
[[Category: Du Q]]
 
[[Category: Egli M]]
==About this Structure==
[[Category: Huang Z]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].  
[[Category: Teplova M]]
 
[[Category: Tereshko V]]
==Reference==
[[Category: Wawrzak Z]]
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12458077 12458077]
[[Category: Wilds CJ]]
[[Category: Carrasco, N.]]
[[Category: Du, Q.]]
[[Category: Egli, M.]]
[[Category: Huang, Z.]]
[[Category: Teplova, M.]]
[[Category: Tereshko, V.]]
[[Category: Wawrzak, Z.]]
[[Category: Wilds, C J.]]
[[Category: 2'-methylseleno-uridine]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:49:12 2008''

Latest revision as of 07:42, 14 February 2024

A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine

1ma8, resolution 1.30Å

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