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New page: left|200px<br /> <applet load="1mfq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mfq, resolution 3.10Å" /> '''Crystal Structure A...
 
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[[Image:1mfq.gif|left|200px]]<br />
<applet load="1mfq" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mfq, resolution 3.10&Aring;" />
'''Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle'''<br />


==Overview==
==Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle==
The eukaryotic signal recognition particle (SRP) is a cytoplasmic, ribonucleoprotein particle that targets secretory and membrane proteins to, the endoplasmic reticulum. The binding of SRP54 to the S domain of 7SL RNA, is highly dependent on SRP19. Here we present the crystal structure of a, human SRP ternary complex consisting of SRP19, the M domain of SRP54 and, the S domain of 7SL RNA. Upon binding of the M domain of SRP54 to the 7SL, RNA-SRP19 complex, the asymmetric loop of helix 8 in 7SL RNA collapses., The bases of the four nucleotides in the long strand of the asymmetric, loop continuously stack and interact with the M domain, whereas the two, adenines in the short strand flip out and form two A-minor motifs with, helix 6. This stabilizing interaction is only possible when helix 6 has, been positioned parallel to helix 8 by the prior binding of SRP19 to the, tetraloops of helices 6 and 8. Hence, the crystal structure of the ternary, complex suggests why SRP19 is necessary for the stable binding of SRP54 to, the S domain RNA.
<StructureSection load='1mfq' size='340' side='right'caption='[[1mfq]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mfq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MFQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mfq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mfq OCA], [https://pdbe.org/1mfq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mfq RCSB], [https://www.ebi.ac.uk/pdbsum/1mfq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mfq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SRP19_HUMAN SRP19_HUMAN] Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mf/1mfq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mfq ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MFQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MFQ OCA].
*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Induced structural changes of 7SL RNA during the assembly of human signal recognition particle., Kuglstatter A, Oubridge C, Nagai K, Nat Struct Biol. 2002 Oct;9(10):740-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12244299 12244299]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Kuglstatter, A.]]
[[Category: Kuglstatter A]]
[[Category: Nagai, K.]]
[[Category: Nagai K]]
[[Category: Oubridge, C.]]
[[Category: Oubridge C]]
[[Category: CL]]
[[Category: MG]]
[[Category: 3-helix junction]]
[[Category: a-minor motif]]
[[Category: rna-protein complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:10:47 2007''

Latest revision as of 07:43, 14 February 2024

Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle

1mfq, resolution 3.10Å

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