8e8w

Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 mononuclear Fe(II) structure on the HDO cofactor assembly pathway

spinqualitylabelsall models 8e8w, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8e8w is a 2 chain structure with sequence from Streptomyces achromogenes subsp. streptozoticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: FE2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SZNF_STRC2 Involved in the biosynthesis of the glucosamine-nitrosourea antibiotic streptozotocin (SZN) (PubMed:30728519, PubMed:30763082). Catalyzes a complex multi-step reaction: the overall reaction is an oxidative rearrangement of the guanidine group of N(omega)-methyl-L-arginine (L-NMA), generating an N-nitrosourea product (PubMed:30728519, PubMed:30763082, PubMed:32511919). SznF first hydroxylates L-NMA to form N(delta)-hydroxy-N(omega)-methyl-L-arginine (L-HMA), which is further hydroxylated to give N(delta)-hydroxy-N(omega)-hydroxy-N(omega)-methyl-L-arginine (L-DHMA) (PubMed:30728519, PubMed:32511919). Subsequently, an oxidative rearrangement converts this intermediate to N(delta)-hydroxy-N(omega)-methyl-N(omega)-nitroso-L-citrulline (PubMed:30728519, PubMed:34004115). This product is unstable, and degrades non-enzymically into nitric oxide and the denitrosated product N(delta)-hydroxy-N(omega)-methyl-L-citrulline (PubMed:30728519).

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