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[[Image:1mgw.gif|left|200px]]
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{{STRUCTURE_1mgw|  PDB=1mgw  |  SCENE=  }}
'''Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease'''


==Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease==
<StructureSection load='1mgw' size='340' side='right'caption='[[1mgw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mgw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MGW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mgw OCA], [https://pdbe.org/1mgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mgw RCSB], [https://www.ebi.ac.uk/pdbsum/1mgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mgw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNS3_KITAU RNS3_KITAU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mgw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mgw ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the 3' side of guanosine residues. Here, x-ray diffraction analysis was used to determine the three-dimensional structure of two distinct crystalline forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures are similar to each other as well as to that of a homolog, RNase Sa. All of the key active-site residues of RNase Sa (Asn(42), Glu(44), Glu(57), Arg(72), and His(88)) are located in the putative active site of RNase Sa3. Also herein, RNase Sa3 is shown to be toxic to human erythroleukemia cells in culture. Like onconase, which is an amphibian ribonuclease in Phase III clinical trials as a cancer chemotherapeutic, RNase Sa3 is not inhibited by the cytosolic ribonuclease inhibitor protein. Thus, a prokaryotic ribonuclease can be toxic to mammalian cells.


==Overview==
X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity.,Sevcik J, Urbanikova L, Leland PA, Raines RT J Biol Chem. 2002 Dec 6;277(49):47325-30. Epub 2002 Sep 11. PMID:12228255<ref>PMID:12228255</ref>
Ribonuclease (RNase) Sa3 is secreted by the Gram-positive bacterium Streptomyces aureofaciens. The enzyme catalyzes the cleavage of RNA on the 3' side of guanosine residues. Here, x-ray diffraction analysis was used to determine the three-dimensional structure of two distinct crystalline forms of RNase Sa3 to a resolution of 2.0 and 1.7 A. These two structures are similar to each other as well as to that of a homolog, RNase Sa. All of the key active-site residues of RNase Sa (Asn(42), Glu(44), Glu(57), Arg(72), and His(88)) are located in the putative active site of RNase Sa3. Also herein, RNase Sa3 is shown to be toxic to human erythroleukemia cells in culture. Like onconase, which is an amphibian ribonuclease in Phase III clinical trials as a cancer chemotherapeutic, RNase Sa3 is not inhibited by the cytosolic ribonuclease inhibitor protein. Thus, a prokaryotic ribonuclease can be toxic to mammalian cells.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1MGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGW OCA].
</div>
<div class="pdbe-citations 1mgw" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity., Sevcik J, Urbanikova L, Leland PA, Raines RT, J Biol Chem. 2002 Dec 6;277(49):47325-30. Epub 2002 Sep 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12228255 12228255]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
[[Category: Single protein]]
== References ==
[[Category: Streptomyces aureofaciens]]
<references/>
[[Category: Leland, P A.]]
__TOC__
[[Category: Raines, R T.]]
</StructureSection>
[[Category: Sevcik, J.]]
[[Category: Kitasatospora aureofaciens]]
[[Category: Urbanikova, L.]]
[[Category: Large Structures]]
[[Category: Alpha/beta protein]]
[[Category: Leland PA]]
[[Category: Ub roll]]
[[Category: Raines RT]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:00:20 2008''
[[Category: Sevcik J]]
[[Category: Urbanikova L]]

Latest revision as of 00:15, 21 November 2024

Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease

1mgw, resolution 2.00Å

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