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New page: left|200px<br /> <applet load="1mkp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mkp, resolution 2.35Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1mkp.gif|left|200px]]<br />
<applet load="1mkp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mkp, resolution 2.35&Aring;" />
'''CRYSTAL STRUCTURE OF PYST1 (MKP3)'''<br />


==Overview==
==CRYSTAL STRUCTURE OF PYST1 (MKP3)==
The crystal structure of the catalytic domain from the MAPK phosphatase, Pyst1 (Pyst1-CD) has been determined at 2.35 A. The structure adopts a, protein tyrosine phosphatase (PTPase) fold with a shallow active site that, displays a distorted geometry in the absence of its substrate with some, similarity to the dual-specificity phosphatase cdc25. Functional, characterization of Pyst1-CD indicates it is sufficient to dephosphorylate, activated ERK2 in vitro. Kinetic analysis of Pyst1 and Pyst1-CD using the, substrate p-nitrophenyl phosphate (pNPP) reveals that both molecules, undergo catalytic activation in the presence of recombinant inactive ERK2, switching from a low- to high-activity form. Mutation of Asp 262, located, 5.5 A distal to the active site, demonstrates it is essential for, catalysis in the high-activity ERK2-dependent conformation of Pyst1 but, not for the low-activity ERK2-independent form, suggesting that ERK2, induces closure of the Asp 262 loop over the active site, thereby, enhancing Pyst1 catalytic efficiency.
<StructureSection load='1mkp' size='340' side='right'caption='[[1mkp]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mkp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MKP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mkp OCA], [https://pdbe.org/1mkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mkp RCSB], [https://www.ebi.ac.uk/pdbsum/1mkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mkp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DUS6_HUMAN DUS6_HUMAN] Inactivates MAP kinases. Has a specificity for the ERK family.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mkp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mkp ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MKP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CL and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MKP OCA].
*[[MAP kinase phosphatase|MAP kinase phosphatase]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of the MAPK phosphatase Pyst1 catalytic domain and implications for regulated activation., Stewart AE, Dowd S, Keyse SM, McDonald NQ, Nat Struct Biol. 1999 Feb;6(2):174-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048930 10048930]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dowd, S.]]
[[Category: Dowd S]]
[[Category: Keyse, S.]]
[[Category: Keyse S]]
[[Category: Mcdonald, N.Q.]]
[[Category: Mcdonald NQ]]
[[Category: Stewart, A.E.]]
[[Category: Stewart AE]]
[[Category: CL]]
[[Category: MPD]]
[[Category: hydrolase]]
 
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Retrieved from "https://proteopedia.org/1mkp"