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New page: left|200px<br /> <applet load="1n3y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3y, resolution 1.65Å" /> '''Crystal structure o...
 
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[[Image:1n3y.gif|left|200px]]<br />
<applet load="1n3y" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n3y, resolution 1.65&Aring;" />
'''Crystal structure of the alpha-X beta2 integrin I domain'''<br />


==Overview==
==Crystal structure of the alpha-X beta2 integrin I domain==
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through, the I domain of the alpha X subunit. Ligands include the complement factor, fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on, other leukocytes, suggests a role in the immune response. We now report, the structure of the alpha X I domain resolved at 1.65 A by x-ray, crystallography. To analyze structural requirements for ligand binding we, made a mutation in the alpha X I domain C-terminal helix, which increased, the affinity for iC3b approximately 200-fold to 2.4 microM compared with, the wild-type domain affinity of approximately 400 microM. Gel permeation, chromatography supported a conformational change between the wild-type and, mutated domains. Conservation of allosteric regulation in the alpha X I, domain points to the functional importance of this phenomenon.
<StructureSection load='1n3y' size='340' side='right'caption='[[1n3y]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n3y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3Y FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3y OCA], [https://pdbe.org/1n3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3y RCSB], [https://www.ebi.ac.uk/pdbsum/1n3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3y ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ITAX_HUMAN ITAX_HUMAN] Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3y_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3y ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1N3Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N3Y OCA].
*[[Integrin 3D structures|Integrin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure and allosteric regulation of the alpha X beta 2 integrin I domain., Vorup-Jensen T, Ostermeier C, Shimaoka M, Hommel U, Springer TA, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1873-8. Epub 2003 Jan 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12554829 12554829]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hommel, U.]]
[[Category: Hommel U]]
[[Category: Ostermeier, C.]]
[[Category: Ostermeier C]]
[[Category: Shimaoka, M.]]
[[Category: Shimaoka M]]
[[Category: Springer, T.A.]]
[[Category: Springer TA]]
[[Category: Vorup-Jensen, T.]]
[[Category: Vorup-Jensen T]]
[[Category: alpha/beta rossmann fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:17:30 2007''

Latest revision as of 07:52, 14 February 2024

Crystal structure of the alpha-X beta2 integrin I domain

1n3y, resolution 1.65Å

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