8u95: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "8u95" [edit=sysop:move=sysop] |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==The structure of myosin heavy chain from Drosophila melanogaster flight muscle thick filaments== | ||
<StructureSection load='8u95' size='340' side='right'caption='[[8u95]], [[Resolution|resolution]] 4.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8u95]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8U95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8U95 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8u95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8u95 OCA], [https://pdbe.org/8u95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8u95 RCSB], [https://www.ebi.ac.uk/pdbsum/8u95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8u95 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/M9ND95_DROME M9ND95_DROME] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Striated muscle thick filaments are composed of myosin II and several non-myosin proteins which define the filament length and modify its function. Myosin II has a globular N-terminal motor domain comprising its catalytic and actin-binding activities and a long alpha-helical, coiled tail that forms the dense filament backbone. Myosin alone polymerizes into filaments of irregular length, but striated muscle thick filaments have defined lengths that, with thin filaments, define the sarcomere structure. The motor domain structure and function are well understood, but the myosin filament backbone is not. Here we report on the structure of the flight muscle thick filaments from Drosophila melanogaster at 4.7 A resolution, which eliminates previous ambiguities in non-myosin densities. The full proximal S2 region is resolved, as are the connecting densities between the Ig domains of stretchin-klp. The proteins, flightin, and myofilin are resolved in sufficient detail to build an atomic model based on an AlphaFold prediction. Our results suggest a method by which flightin and myofilin cooperate to define the structure of the thick filament and explains a key myosin mutation that affects flightin incorporation. Drosophila is a genetic model organism for which our results can define strategies for functional testing. | |||
Structure of the Drosophila melanogaster Flight Muscle Myosin Filament at 4.7 A Resolution Reveals New Details of Non-Myosin Proteins.,Abbasi Yeganeh F, Rastegarpouyani H, Li J, Taylor KA Int J Mol Sci. 2023 Oct 5;24(19):14936. doi: 10.3390/ijms241914936. PMID:37834384<ref>PMID:37834384</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8u95" style="background-color:#fffaf0;"></div> | ||
[[Category: Li | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | |||
[[Category: Abbasi Yeganeh F]] | |||
[[Category: Li J]] | |||
[[Category: Rastegarpouyani H]] | |||
[[Category: Taylor KA]] | |||
Latest revision as of 06:23, 14 May 2025
The structure of myosin heavy chain from Drosophila melanogaster flight muscle thick filaments
| ||||||||||||
