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New page: '''Unreleased structure''' The entry 8wkh is ON HOLD Authors: Zhu, K.L., Gong, Y., Cui, Y.B. Description: Crystal structure of group 13 allergen from Blomia tropicalis [[Category: Unre...
 
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'''Unreleased structure'''


The entry 8wkh is ON HOLD
==Crystal structure of group 13 allergen from Blomia tropicalis==
<StructureSection load='8wkh' size='340' side='right'caption='[[8wkh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wkh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WKH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wkh OCA], [https://pdbe.org/8wkh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wkh RCSB], [https://www.ebi.ac.uk/pdbsum/8wkh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wkh ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Blomia tropicalis is an important species of allergenic mite. Structurally related cross-reactive allergens are involved in pathogenesis of clinical symptoms. The present study focused on recombinant allergen rBlo t 13 from B. tropicalis, including investigation of its structure, immunological properties, IgE-mediated cross-reactivity. In this work, the prokaryotic expression plasmids pET-28(a)-Blo t 13, pET-28(a)-Der f 13, and pET-28(a)-Tyr p 13 were constructed, transformed into E. coli Rosetta (DE3) pLysS, and purified by nickel affinity chromatography, respectively. By using ELISA, the IgE-binding rates were detected for rBlo t 13 and its epitope peptides, as well as the cross-reactivity among rBlo t 13, rDer f 13, and rTyr p 13. The tertiary structure of rBlo t 13 was resolved using X-ray diffraction at 2.0 A resolution. Using IgE-ELISA, the IgE binding rate of rBlo t 13 was 60 % with Blomia tropicalis-positive sera. In the experiments of ELISA for cross-reactivity with rBlo t 13 on solid phase, the inhibition rates were 65 %, 57 % and 63 % for rBlo t 13, rDer f 13, and rTyr p 13, respectively. The structure of Blo t 13 protein contains a beta-barrel structure which is composed of 10 beta strands and has 2 alpha helices at the end of the barrel. Comparison of the tertiary structures of rBlo t 13, rDer f 13, and rTyr p 13 revealed that the beta-barrel structure is highly conserved, consistent with the alignment of amino acid sequences. We obtained the recombinant protein rBlo t 13, demonstrated its cross-reactivity with Der f 13 and Tyr p 13 due to their structural similarity.


Authors: Zhu, K.L., Gong, Y., Cui, Y.B.
Immunobiological properties and structure analysis of group 13 allergen from Blomia tropicalis and its IgE-mediated cross-reactivity.,Zhou Y, Zhu K, Li Q, Zhou D, Ren Y, Liao Y, Cao P, Gong Y, Cui Y Int J Biol Macromol. 2023 Nov 4;254(Pt 3):127788. doi: , 10.1016/j.ijbiomac.2023.127788. PMID:37926306<ref>PMID:37926306</ref>


Description: Crystal structure of group 13 allergen from Blomia tropicalis
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Zhu, K.L]]
<div class="pdbe-citations 8wkh" style="background-color:#fffaf0;"></div>
[[Category: Cui, Y.B]]
== References ==
[[Category: Gong, Y]]
<references/>
__TOC__
</StructureSection>
[[Category: Blomia tropicalis]]
[[Category: Large Structures]]
[[Category: Cui YB]]
[[Category: Gong Y]]
[[Category: Zhu KL]]
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