1n7f: Difference between revisions

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[[Image:1n7f.jpg|left|200px]]


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==Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide==
The line below this paragraph, containing "STRUCTURE_1n7f", creates the "Structure Box" on the page.
<StructureSection load='1n7f' size='340' side='right'caption='[[1n7f]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1n7f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7F FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7f OCA], [https://pdbe.org/1n7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7f RCSB], [https://www.ebi.ac.uk/pdbsum/1n7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7f ProSAT]</span></td></tr>
{{STRUCTURE_1n7f|  PDB=1n7f  |  SCENE= }}
</table>
 
== Function ==
'''Crystal structure of the sixth PDZ domain of GRIP1 in complex with liprin C-terminal peptide'''
[https://www.uniprot.org/uniprot/LIPA1_HUMAN LIPA1_HUMAN] May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.<ref>PMID:7796809</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
PDZ domains bind to short segments within target proteins in a sequence-specific fashion. Glutamate receptor-interacting protein (GRIP)/ABP family proteins contain six to seven PDZ domains and interact via the sixth PDZ domain (class II) with the C termini of various proteins including liprin-alpha. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class II PDZ domain and PDZ-mediated multimerization, we determined the crystal structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8 A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix alphaB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7f_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1N7F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7F OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7f ConSurf].
Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12493751 12493751]
<div style="clear:both"></div>
[[Category: Protein complex]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Eom, S H.]]
[[Category: Eom SH]]
[[Category: Im, Y J.]]
[[Category: Im YJ]]
[[Category: Kang, G B.]]
[[Category: Kang GB]]
[[Category: Kim, E.]]
[[Category: Kim E]]
[[Category: Lee, J H.]]
[[Category: Lee JH]]
[[Category: Park, S H.]]
[[Category: Park SH]]
[[Category: Rho, S H.]]
[[Category: Rho SH]]
[[Category: Sheng, M.]]
[[Category: Sheng M]]
[[Category: Grip]]
[[Category: Liprin]]
[[Category: Pdz]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:11:14 2008''
Retrieved from "https://proteopedia.org/1n7f"