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[[Image:1o7i.jpg|left|200px]]
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{{STRUCTURE_1o7i|  PDB=1o7i  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN'''


==Crystal structure of a single stranded DNA binding protein==
<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [https://pdbe.org/1o7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [https://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.


==Overview==
Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373<ref>PMID:12773373</ref>
Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1O7I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA].
</div>
<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein., Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF, EMBO J. 2003 Jun 2;22(11):2561-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12773373 12773373]
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
[[Category: Single protein]]
== References ==
[[Category: Sulfolobus solfataricus]]
<references/>
[[Category: Kerr, I D.]]
__TOC__
[[Category: Naismith, J H.]]
</StructureSection>
[[Category: Dna-binding protein]]
[[Category: Large Structures]]
[[Category: Ob fold]]
[[Category: Saccharolobus solfataricus P2]]
[[Category: Single stranded dna]]
[[Category: Kerr ID]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:29:12 2008''
[[Category: Naismith JH]]
Retrieved from "https://proteopedia.org/1o7i"