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New page: left|200px<br /> <applet load="1p9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9f" /> '''NMR Structure of Neurokinin B from DYANA'''...
 
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[[Image:1p9f.gif|left|200px]]<br />
<applet load="1p9f" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1p9f" />
'''NMR Structure of Neurokinin B from DYANA'''<br />


==Overview==
==NMR Structure of Neurokinin B from DYANA==
Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety, of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain, insight into structure-function relationship, three-dimensional structure, of NKB has been investigated using CD spectropolarimetry and, two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy, in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of, resonances have been made with the aid of correlation spectroscopy, (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect, Spectroscopy (NOESY) experiments. Distance constraints obtained from the, NMR data have been used to generate a family of structures, which have, been refined using restrained energy minimization and dynamics. Our data, show that a helical structure is induced in NKB, in presence of, perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model, system. Further, the conformation adopted by NKB in presence of DPC, micelles represents a structural motif typical of neurokinin-3 selective, agonists.
<StructureSection load='1p9f' size='340' side='right'caption='[[1p9f]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1p9f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9f OCA], [https://pdbe.org/1p9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9f RCSB], [https://www.ebi.ac.uk/pdbsum/1p9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TKNK_HUMAN TKNK_HUMAN] Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.


==About this Structure==
Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles.,Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:15317475<ref>PMID:15317475</ref>
1P9F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P9F OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Three dimensional structure of mammalian tachykinin peptide neurokinin B bound to lipid micelles., Mantha AK, Chandrashekar IR, Baquer NZ, Cowsik SM, J Biomol Struct Dyn. 2004 Oct;22(2):137-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15317475 15317475]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1p9f" style="background-color:#fffaf0;"></div>
[[Category: Baquer, N.Z.]]
== References ==
[[Category: Chandrashekar, I.R.]]
<references/>
[[Category: Cowsik, S.M.]]
__TOC__
[[Category: Mantha, A.K.]]
</StructureSection>
[[Category: neuropeptide]]
[[Category: Homo sapiens]]
[[Category: protein]]
[[Category: Large Structures]]
[[Category: structures from dyana]]
[[Category: Baquer NZ]]
 
[[Category: Chandrashekar IR]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:41:25 2007''
[[Category: Cowsik SM]]
[[Category: Mantha AK]]
Retrieved from "https://proteopedia.org/1p9f"