4emq

Crystal structure of a single mutant of Dronpa, the green-on-state PDM1-4

spinqualitylabelsall models 4emq, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4emq is a 6 chain structure with sequence from Echinophyllia sp. SC22. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Ligands: 1PE, EPE, GYC, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q5TLG6_9CNID Publication Abstract from PubMed The crystal structure of the on-state of PDM1-4, a single-mutation variant of the photochromic fluorescent protein Dronpa, is reported at 1.95 A resolution. PDM1-4 is a Dronpa variant that possesses a slower off-switching rate than Dronpa and thus can effectively increase the image resolution in subdiffraction optical microscopy, although the precise molecular basis for this change has not been elucidated. This work shows that the Lys145Asn mutation in PDM1-4 stabilizes the interface available for dimerization, facilitating oligomerization of the protein. No significant changes were observed in the chromophore environment of PDM1-4 compared with Dronpa, and the ensemble absorption and emission properties of PDM1-4 were highly similar to those of Dronpa. It is proposed that the slower off-switching rate in PDM1-4 is caused by a decrease in the potential flexibility of certain beta-strands caused by oligomerization along the AC interface. Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa.,Nguyen Bich N, Moeyaert B, Van Hecke K, Dedecker P, Mizuno H, Hofkens J, Van Meervelt L Acta Crystallogr D Biol Crystallogr. 2012 Dec;68(Pt 12):1653-9. doi:, 10.1107/S0907444912039686. Epub 2012 Nov 9. PMID:23151630 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dronpa

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