1prx: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
New page: left|200px<br /> <applet load="1prx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1prx, resolution 2.0Å" /> '''HORF6 A NOVEL HUMAN ...
 
OCA (talk | contribs)
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1prx.gif|left|200px]]<br />
<applet load="1prx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1prx, resolution 2.0&Aring;" />
'''HORF6 A NOVEL HUMAN PEROXIDASE ENZYME'''<br />


==Overview==
==HORF6 A NOVEL HUMAN PEROXIDASE ENZYME==
Hydrogen peroxide (H2O2) has been implicated recently as an intracellular, messenger that affects cellular processes including protein, phosphorylation, transcription and apoptosis. A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of, H2O2 by reducing it in the presence of an appropriate electron donor. The, crystal structure of a human Prx enzyme, hORF6, reveals that the protein, contains two discrete domains and forms a dimer. The N-terminal domain has, a thioredoxin fold and the C-terminal domain is used for dimerization. The, active site cysteine (Cys 47), which exists as cysteine-sulfenic acid in, the crystal, is located at the bottom of a relatively narrow pocket. The, positively charged environment surrounding Cys 47 accounts for the, peroxidase activity of the enzyme, which contains no redox cofactors.
<StructureSection load='1prx' size='340' side='right'caption='[[1prx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1prx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1prx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1prx OCA], [https://pdbe.org/1prx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1prx RCSB], [https://www.ebi.ac.uk/pdbsum/1prx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1prx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRDX6_HUMAN PRDX6_HUMAN] Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1prx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1prx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hydrogen peroxide (H2O2) has been implicated recently as an intracellular messenger that affects cellular processes including protein phosphorylation, transcription and apoptosis. A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the presence of an appropriate electron donor. The crystal structure of a human Prx enzyme, hORF6, reveals that the protein contains two discrete domains and forms a dimer. The N-terminal domain has a thioredoxin fold and the C-terminal domain is used for dimerization. The active site cysteine (Cys 47), which exists as cysteine-sulfenic acid in the crystal, is located at the bottom of a relatively narrow pocket. The positively charged environment surrounding Cys 47 accounts for the peroxidase activity of the enzyme, which contains no redox cofactors.


==About this Structure==
Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution.,Choi HJ, Kang SW, Yang CH, Rhee SG, Ryu SE Nat Struct Biol. 1998 May;5(5):400-6. PMID:9587003<ref>PMID:9587003</ref>
1PRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PRX OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution., Choi HJ, Kang SW, Yang CH, Rhee SG, Ryu SE, Nat Struct Biol. 1998 May;5(5):400-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9587003 9587003]
</div>
<div class="pdbe-citations 1prx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Choi, H.J.]]
[[Category: Choi H-J]]
[[Category: Kang, S.W.]]
[[Category: Kang SW]]
[[Category: Rhee, S.G.]]
[[Category: Rhee SG]]
[[Category: Ryu, S.E.]]
[[Category: Ryu S-E]]
[[Category: Yang, C.H.]]
[[Category: Yang C-H]]
[[Category: antioxidant]]
[[Category: cellular signaling]]
[[Category: horf6]]
[[Category: hydrogen peroxide]]
[[Category: peroxiredoxin]]
[[Category: redox regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:46:34 2007''

Latest revision as of 07:13, 30 October 2024

HORF6 A NOVEL HUMAN PEROXIDASE ENZYME

1prx, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA