8v53: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "8v53" [edit=sysop:move=sysop] |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==CryoEM structure of Ku homodimer in complex with hairpin DNA== | |||
<StructureSection load='8v53' size='340' side='right'caption='[[8v53]], [[Resolution|resolution]] 4.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8v53]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V53 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1Å</td></tr> | |||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v53 OCA], [https://pdbe.org/8v53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v53 RCSB], [https://www.ebi.ac.uk/pdbsum/8v53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v53 ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/KU_MYCTU KU_MYCTU] With LigD forms a non-homologous end joining (NHEJ) repair enzyme. Binds linear dsDNA with 5'- and 3'-overhangs but not closed circular dsDNA nor ssDNA. One dimer binds for every 30 bp. Recruits and stimulates the ligase activity of LigD but not of T4 ligase or a human ligase complex (LIG4/XRCC4). Attenuates the 3'- to 5'-exonuclease activity of LigD. Stimulates the template-directed addition of dNTPs by LigD on 5'-overhangs and nuclease activity on 3'-overhangs.<ref>PMID:12215643</ref> <ref>PMID:15499016</ref> <ref>PMID:15778718</ref> <ref>PMID:16023671</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycobacterium tuberculosis H37Rv]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Baral J]] | |||
[[Category: Das AK]] | |||
[[Category: Rouiller I]] | |||
Latest revision as of 08:00, 11 December 2025
CryoEM structure of Ku homodimer in complex with hairpin DNA
| ||||||||||||
