8v9o: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "8v9o" [edit=sysop:move=sysop] |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Imaging scaffold engineered to bind the therapeutic protein target BARD1== | ||
<StructureSection load='8v9o' size='340' side='right'caption='[[8v9o]], [[Resolution|resolution]] 3.81Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8v9o]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V9O FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.81Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v9o OCA], [https://pdbe.org/8v9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v9o RCSB], [https://www.ebi.ac.uk/pdbsum/8v9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v9o ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Imaging scaffolds composed of designed protein cages fused to designed ankyrin repeat proteins (DARPins) have enabled the structure determination of small proteins by cryogenic electron microscopy (cryo-EM). One particularly well characterized scaffold type is a symmetric tetrahedral assembly composed of 24 subunits, 12 A and 12 B, which has three cargo-binding DARPins positioned on each vertex. Here, the X-ray crystal structure of a representative tetrahedral scaffold in the apo state is reported at 3.8 A resolution. The X-ray crystal structure complements recent cryo-EM findings on a closely related scaffold, while also suggesting potential utility for crystallographic investigations. As observed in this crystal structure, one of the three DARPins, which serve as modular adaptors for binding diverse ;cargo' proteins, present on each of the vertices is oriented towards a large solvent channel. The crystal lattice is unusually porous, suggesting that it may be possible to soak crystals of the scaffold with small (</=30 kDa) protein cargo ligands and subsequently determine cage-cargo structures via X-ray crystallography. The results suggest the possibility that cryo-EM scaffolds may be repurposed for structure determination by X-ray crystallography, thus extending the utility of electron-microscopy scaffold designs for alternative structural biology applications. | |||
X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation.,Agdanowski MP, Castells-Graells R, Sawaya MR, Cascio D, Yeates TO, Arbing MA Acta Crystallogr F Struct Biol Commun. 2024 May 1;80(Pt 5):107-115. doi: , 10.1107/S2053230X2400414X. Epub 2024 May 20. PMID:38767964<ref>PMID:38767964</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Agdanowski | <div class="pdbe-citations 8v9o" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Agdanowski MP]] | |||
[[Category: Arbing MA]] | |||
[[Category: Castells-Graells R]] | |||
[[Category: Sawaya MR]] | |||
[[Category: Yeates TO]] | |||
