8vci: Difference between revisions
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New page: '''Unreleased structure''' The entry 8vci is ON HOLD Authors: Peterson, J.M., Becker, S.T., O''Leary, C.A., Juneja, P., Yang, Y., Moss, W.N. Description: SARS-CoV-2 Frameshift Stimulat... |
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The | ==SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop== | ||
<StructureSection load='8vci' size='340' side='right'caption='[[8vci]], [[Resolution|resolution]] 6.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8vci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VCI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vci OCA], [https://pdbe.org/8vci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vci RCSB], [https://www.ebi.ac.uk/pdbsum/8vci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vci ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshift stimulatory element (FSE) is necessary for programmed -1 ribosomal frameshifting (-1 PRF) and optimized viral efficacy. The FSE has an abundance of context-dependent alternate conformations, but two of the structures most crucial to -1 PRF are an attenuator hairpin and a three-stem H-type pseudoknot structure. A crystal structure of the pseudoknot alone features three RNA stems in a helically stacked linear structure, whereas a 6.9 A cryo-EM structure including the upstream heptameric slippery site resulted in a bend between two stems. Our previous research alluded to an extended upstream multibranch loop that includes both the attenuator hairpin and the slippery site-a conformation not previously modeled. We aim to provide further context to the SARS-CoV-2 FSE via computational and medium resolution cryo-EM approaches, by presenting a 6.1 A cryo-EM structure featuring a linear pseudoknot structure and a dynamic upstream multibranch loop. | |||
Structure of the SARS-CoV-2 Frameshift Stimulatory Element with an Upstream Multibranch Loop.,Peterson JM, Becker ST, O'Leary CA, Juneja P, Yang Y, Moss WN Biochemistry. 2024 May 21;63(10):1287-1296. doi: 10.1021/acs.biochem.3c00716. , Epub 2024 May 10. PMID:38727003<ref>PMID:38727003</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8vci" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Moss | __TOC__ | ||
[[Category: O | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Severe acute respiratory syndrome coronavirus 2]] | |||
[[Category: Becker ST]] | |||
[[Category: Juneja P]] | |||
[[Category: Moss WN]] | |||
[[Category: O'Leary CA]] | |||
[[Category: Peterson JM]] | |||
[[Category: Yang Y]] | |||
Latest revision as of 19:58, 29 May 2024
SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop
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