1qjb: Difference between revisions

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New page: left|200px<br /> <applet load="1qjb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qjb, resolution 2.0Å" /> '''14-3-3 ZETA/PHOSPHOP...
 
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[[Image:1qjb.gif|left|200px]]<br />
<applet load="1qjb" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1qjb, resolution 2.0&Aring;" />
'''14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)'''<br />


==Overview==
==14-3-3 ZETA/PHOSPHOPEPTIDE COMPLEX (MODE 1)==
We have solved the high-resolution X-ray structure of 14-3-3 bound to two, different phosphoserine peptides, representing alternative, substrate-binding motifs. These structures reveal an evolutionarily, conserved network of peptide-protein interactions within all 14-3-3, isotypes, explain both binding motifs, and identify a novel intrachain, phosphorylation-mediated loop structure in one of the peptides. A 14-3-3, mutation disrupting Raf signaling alters the ligand-binding cleft, selecting a different phosphopeptide-binding motif and different, substrates than the wild-type protein. Many 14-3-3: peptide contacts, involve a C-terminal amphipathic alpha helix containing a putative nuclear, export signal, implicating this segment in both ligand and Crm1 binding., Structural homology between the 14-3-3 NES structure and those within I, kappa B alpha and p53 reveals a conserved topology recognized by the Crm1, nuclear export machinery.
<StructureSection load='1qjb' size='340' side='right'caption='[[1qjb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qjb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=14ps 14ps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QJB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qjb OCA], [https://pdbe.org/1qjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qjb RCSB], [https://www.ebi.ac.uk/pdbsum/1qjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qjb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/1433Z_HUMAN 1433Z_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.<ref>PMID:9360956</ref> <ref>PMID:14578935</ref> <ref>PMID:15071501</ref> <ref>PMID:15644438</ref> <ref>PMID:16376338</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qj/1qjb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qjb ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1QJB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure superseeds the now removed PDB entry 14PS. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJB OCA].
*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
 
== References ==
==Reference==
<references/>
Structural analysis of 14-3-3 phosphopeptide complexes identifies a dual role for the nuclear export signal of 14-3-3 in ligand binding., Rittinger K, Budman J, Xu J, Volinia S, Cantley LC, Smerdon SJ, Gamblin SJ, Yaffe MB, Mol Cell. 1999 Aug;4(2):153-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10488331 10488331]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Budman, J.]]
[[Category: Budman J]]
[[Category: Cantley, L.C.]]
[[Category: Cantley LC]]
[[Category: Gamblin, S.J.]]
[[Category: Gamblin SJ]]
[[Category: Rittinger, K.]]
[[Category: Rittinger K]]
[[Category: Smerdon, S.J.]]
[[Category: Smerdon SJ]]
[[Category: Volinia, S.]]
[[Category: Volinia S]]
[[Category: Xu, J.]]
[[Category: Xu J]]
[[Category: Yaffe, M.B.]]
[[Category: Yaffe MB]]
[[Category: 14-3-3]]
[[Category: complex (peptide)]]
[[Category: phosphopeptide]]
[[Category: signal transducti]]
 
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Retrieved from "https://proteopedia.org/1qjb"