8xgr: Difference between revisions
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==ETB-eGt complex bound to endothelin-1== | |||
<StructureSection load='8xgr' size='340' side='right'caption='[[8xgr]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8xgr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Lama_glama Lama glama] and [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XGR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xgr OCA], [https://pdbe.org/8xgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xgr RCSB], [https://www.ebi.ac.uk/pdbsum/8xgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xgr ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cryo-EM single particle analysis has recently facilitated the high-resolution structural determination of numerous GPCR-G complexes. Diverse methodologies have been devised with this trend, and in the case of GPCR-G(i) complexes, scFv16, an antibody that recognizes the intricate interface of the complex, has been mainly implemented to stabilize the complex. However, owing to their flexibility and heterogeneity, structural determinations of GPCR-G(i) complexes remain both challenging and resource-intensive. By employing eGalpha(t), which exhibits binding affinity to modified nanobody Nb35, the cryo-EM structure of Rhodopsin-eGalpha(t) complex was previously reported. Using this modified G protein, we determined the structure of the ET(B)-eG(t) complex bound to the modified Nb35. The determined structure of ET(B) receptor was the same as the previously reported ET(B)-G(i) complex, and the resulting dataset demonstrated significantly improved anisotropy. This modified G protein will be utilized for the structural determination of other GPCR-G(i) complexes. | |||
Optimizing cryo-EM structural analysis of G(i)-coupling receptors via engineered G(t) and Nb35 application.,Oshima HS, Sano FK, Akasaka H, Iwama A, Shihoya W, Nureki O Biochem Biophys Res Commun. 2024 Jan 22;693:149361. doi: , 10.1016/j.bbrc.2023.149361. Epub 2023 Dec 7. PMID:38128244<ref>PMID:38128244</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8xgr" style="background-color:#fffaf0;"></div> | ||
[[Category: Akasaka | == References == | ||
[[Category: | <references/> | ||
[[Category: Oshima | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Shihoya | [[Category: Bos taurus]] | ||
[[Category: Homo sapiens]] | |||
[[Category: Lama glama]] | |||
[[Category: Large Structures]] | |||
[[Category: Rattus rattus]] | |||
[[Category: Akasaka H]] | |||
[[Category: Iwama A]] | |||
[[Category: Nureki O]] | |||
[[Category: Oshima HS]] | |||
[[Category: Sano FK]] | |||
[[Category: Shihoya W]] | |||
Latest revision as of 10:04, 9 October 2024
ETB-eGt complex bound to endothelin-1
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