8rn0: Difference between revisions

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New page: '''Unreleased structure''' The entry 8rn0 is ON HOLD until sometime in the future Authors: Arragain, B., Cusack, S. Description: Influenza polymerase A/H7N9-4M encapsidase plus 627(R) ...
 
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'''Unreleased structure'''


The entry 8rn0 is ON HOLD  until sometime in the future
==Influenza polymerase A/H7N9-4M encapsidase plus 627(R) / human ANP32A (from "Influenza polymerase A/H7N9-4M replication complex" | Local refinement)==
<StructureSection load='8rn0' size='340' side='right'caption='[[8rn0]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rn0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Zhejiang/DTID-ZJU01/2013(H7N9)) Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RN0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.13&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rn0 OCA], [https://pdbe.org/8rn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rn0 RCSB], [https://www.ebi.ac.uk/pdbsum/8rn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rn0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/X5F427_9INFA X5F427_9INFA] Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex.[HAMAP-Rule:MF_04062][SAAS:SAAS01109044]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Replication of influenza viral RNA depends on at least two viral polymerases, a parental replicase and an encapsidase, and cellular factor ANP32. ANP32 comprises an LRR domain and a long C-terminal low complexity acidic region (LCAR). Here we present evidence suggesting that ANP32 is recruited to the replication complex as an electrostatic chaperone that stabilises the encapsidase moiety within apo-polymerase symmetric dimers that are distinct for influenza A and B polymerases. The ANP32 bound encapsidase, then forms the asymmetric replication complex with the replicase, which is embedded in a parental ribonucleoprotein particle (RNP). Cryo-EM structures reveal the architecture of the influenza A and B replication complexes and the likely trajectory of the nascent RNA product into the encapsidase. The cryo-EM map of the FluB replication complex shows extra density attributable to the ANP32 LCAR wrapping around and stabilising the apo-encapsidase conformation. These structures give new insight into the various mutations that adapt avian strain polymerases to use the distinct ANP32 in mammalian cells.


Authors: Arragain, B., Cusack, S.
Structures of influenza A and B replication complexes give insight into avian to human host adaptation and reveal a role of ANP32 as an electrostatic chaperone for the apo-polymerase.,Arragain B, Krischuns T, Pelosse M, Drncova P, Blackledge M, Naffakh N, Cusack S Nat Commun. 2024 Aug 19;15(1):6910. doi: 10.1038/s41467-024-51007-3. PMID:39160148<ref>PMID:39160148</ref>


Description: Influenza polymerase A/H7N9-4M encapsidase plus 627(R) / human ANP32A (from ""Influenza polymerase A/H7N9-4M replication complex"" | Local refinement)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Arragain, B]]
<div class="pdbe-citations 8rn0" style="background-color:#fffaf0;"></div>
[[Category: Cusack, S]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Arragain B]]
[[Category: Cusack S]]
Retrieved from "https://proteopedia.org/8rn0"