8xyf: Difference between revisions
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New page: '''Unreleased structure''' The entry 8xyf is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal structure of Holo-PlyGRCS, a bacteriophage Endolysin in complex with Cold shock protein C== | |||
<StructureSection load='8xyf' size='340' side='right'caption='[[8xyf]], [[Resolution|resolution]] 1.67Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8xyf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Staphylococcus_phage_GRCS Staphylococcus phage GRCS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XYF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xyf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xyf OCA], [https://pdbe.org/8xyf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xyf RCSB], [https://www.ebi.ac.uk/pdbsum/8xyf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xyf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/W6EBY7_9CAUD W6EBY7_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Staphylococcus aureus causes a wide range of infections, from mild skin conditions to severe, life-threatening diseases. Bacteriophage endolysins exhibit a selective capacity to degrade the peptidoglycan layer of Gram-positive bacteria, making promising biotherapeutic agents against antibiotic-resistant infections. PlyGRCS, a specific endolysin derived from S. aureus, comprises a catalytic CHAP domain and a cell-wall binding SH3_5 domain connected by a linker. Ca(2+) ions are essential for the CHAP domain's catalytic function. The crystal structure of PlyGRCS, determined in the absence of Ca(2+) and refined to a resolution of 1.67 A, revealed significant conformational changes in the Ca(2+) binding site. Antimicrobial assays with Ca(2+)-deficient PlyGRCS and mutants targeting key residues in the catalytic and Ca(2+) binding regions highlighted the importance of specific functional residues for lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). These structural and microbial studies provide valuable insights into the critical residues contributing to PlyGRCS's bacteriolytic efficacy against MRSA. | |||
Structural Basis for the Essential Role of Ca(2+) in the Lytic Activity of Staphylococcus aureus PlyGRCS Endolysin Targeting Methicillin-Resistant Staphylococcus aureus.,Krishnappa G, Nagaraj H, SureshKumar HB, Mandal M, Padavattan S, Bahubali VH, Thiyagarajan S, Padmanabhan B Proteins. 2024 Dec 11. doi: 10.1002/prot.26777. PMID:39660753<ref>PMID:39660753</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8xyf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Staphylococcus phage GRCS]] | |||
[[Category: Gopinath K]] | |||
[[Category: Harshitha HN]] | |||
[[Category: Padmanabhan B]] | |||
Latest revision as of 13:59, 1 January 2025
Crystal structure of Holo-PlyGRCS, a bacteriophage Endolysin in complex with Cold shock protein C
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