8xzo: Difference between revisions
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==Crystal structure of folE riboswitch with Guanine== | |||
<StructureSection load='8xzo' size='340' side='right'caption='[[8xzo]], [[Resolution|resolution]] 1.97Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8xzo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified_eubacterium_clone_A70 Unidentified eubacterium clone A70]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XZO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xzo OCA], [https://pdbe.org/8xzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xzo RCSB], [https://www.ebi.ac.uk/pdbsum/8xzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xzo ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Riboswitches are conserved regulatory RNA elements participating in various metabolic pathways. Recently, a novel RNA motif known as the folE RNA motif was discovered upstream of folE genes. It specifically senses tetrahydrofolate (THF) and is therefore termed THF-II riboswitch. To unravel the ligand recognition mechanism of this newly discovered riboswitch and decipher the underlying principles governing its tertiary folding, we determined both the free-form and bound-form THF-II riboswitch in the wild-type sequences. Combining structural information and isothermal titration calorimetry (ITC) binding assays on structure-based mutants, we successfully elucidated the significant long-range interactions governing the function of THF-II riboswitch and identified additional compounds, including alternative natural metabolites and potential lead compounds for drug discovery, that interact with THF-II riboswitch. Our structural research on the ligand recognition mechanism of the THF-II riboswitch not only paves the way for identification of compounds targeting riboswitches, but also facilitates the exploration of THF analogs in diverse biological contexts or for therapeutic applications. | |||
Structure-based characterization and compound identification of the wild-type THF class-II riboswitch.,Li C, Xu X, Geng Z, Zheng L, Song Q, Shen X, Wu J, Zhao J, Li H, He M, Tai X, Zhang L, Ma J, Dong Y, Ren A Nucleic Acids Res. 2024 May 20:gkae377. doi: 10.1093/nar/gkae377. PMID:38769061<ref>PMID:38769061</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8xzo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Unidentified eubacterium clone A70]] | |||
[[Category: Li CY]] | |||
[[Category: Ren AM]] | |||
Latest revision as of 06:36, 24 July 2024
Crystal structure of folE riboswitch with Guanine
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