1puj: Difference between revisions

Latest revision as of 08:10, 14 February 2024

Structure of B. subtilis YlqF GTPase

Structural highlights

1puj is a 1 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	GNP, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

RBGA_BACSU Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Schaefer L, Uicker WC, Wicker-Planquart C, Foucher AE, Jault JM, Britton RA. Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. J Bacteriol. 2006 Dec;188(23):8252-8. Epub 2006 Sep 22. PMID:16997968 doi:https://dx.doi.org/JB.01213-06
↑ Matsuo Y, Morimoto T, Kuwano M, Loh PC, Oshima T, Ogasawara N. The GTP-binding protein YlqF participates in the late step of 50 S ribosomal subunit assembly in Bacillus subtilis. J Biol Chem. 2006 Mar 24;281(12):8110-7. Epub 2006 Jan 23. PMID:16431913 doi:https://dx.doi.org/M512556200
↑ Uicker WC, Schaefer L, Britton RA. The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis. Mol Microbiol. 2006 Jan;59(2):528-40. PMID:16390447 doi:https://dx.doi.org/MMI4948
↑ Matsuo Y, Oshima T, Loh PC, Morimoto T, Ogasawara N. Isolation and characterization of a dominant negative mutant of Bacillus subtilis GTP-binding protein, YlqF, essential for biogenesis and maintenance of the 50 S ribosomal subunit. J Biol Chem. 2007 Aug 31;282(35):25270-7. Epub 2007 Jul 5. PMID:17613524 doi:https://dx.doi.org/M703894200

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Schaefer L, Uicker WC, Wicker-Planquart C, Foucher AE, Jault JM, Britton RA. Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. J Bacteriol. 2006 Dec;188(23):8252-8. Epub 2006 Sep 22. PMID:16997968 doi:https://dx.doi.org/JB.01213-06

[2] Matsuo Y, Morimoto T, Kuwano M, Loh PC, Oshima T, Ogasawara N. The GTP-binding protein YlqF participates in the late step of 50 S ribosomal subunit assembly in Bacillus subtilis. J Biol Chem. 2006 Mar 24;281(12):8110-7. Epub 2006 Jan 23. PMID:16431913 doi:https://dx.doi.org/M512556200

[3] Uicker WC, Schaefer L, Britton RA. The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis. Mol Microbiol. 2006 Jan;59(2):528-40. PMID:16390447 doi:https://dx.doi.org/MMI4948

[4] Matsuo Y, Oshima T, Loh PC, Morimoto T, Ogasawara N. Isolation and characterization of a dominant negative mutant of Bacillus subtilis GTP-binding protein, YlqF, essential for biogenesis and maintenance of the 50 S ribosomal subunit. J Biol Chem. 2007 Aug 31;282(35):25270-7. Epub 2007 Jul 5. PMID:17613524 doi:https://dx.doi.org/M703894200

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
-[[Image:1puj.gif|left|200px]]
-<!--
+==Structure of B. subtilis YlqF GTPase==
-The line below this paragraph, containing "STRUCTURE_1puj", creates the "Structure Box" on the page.
+<StructureSection load='1puj' size='340' side='right'caption='[[1puj]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1puj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PUJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-{{STRUCTURE_1puj|  PDB=1puj  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1puj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1puj OCA], [https://pdbe.org/1puj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1puj RCSB], [https://www.ebi.ac.uk/pdbsum/1puj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1puj ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1puj TOPSAN]</span></td></tr>
+</table>
-'''Structure of B. subtilis YlqF GTPase'''
+== Function ==
+[https://www.uniprot.org/uniprot/RBGA_BACSU RBGA_BACSU] Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.<ref>PMID:16997968</ref> <ref>PMID:16431913</ref> <ref>PMID:16390447</ref> <ref>PMID:17613524</ref>
+== Evolutionary Conservation ==
-==About this Structure==
+[[Image:Consurf_key_small.gif|200px|right]]
-PUJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUJ OCA].
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pu/1puj_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1puj ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Buglino, J.]]
+[[Category: Buglino J]]
-[[Category: Burley, S K.]]
+[[Category: Burley SK]]
-[[Category: Kniewel, R.]]
+[[Category: Kniewel R]]
-[[Category: Lima, C D.]]
+[[Category: Lima CD]]
-[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
-[[Category: Gtpase]]
-[[Category: New york structural genomix research consortium]]
-[[Category: Nysgxrc t18]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:30:03 2008''

1puj: Difference between revisions

Latest revision as of 08:10, 14 February 2024

Structure of B. subtilis YlqF GTPase

Structural highlights

Function

Evolutionary Conservation

References

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