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[[Image:1pvr.gif|left|200px]]
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{{STRUCTURE_1pvr|  PDB=1pvr  |  SCENE=  }}
'''BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE LOXP (WILDTYPE) RECOGNITION SITE'''


==BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE LOXP (WILDTYPE) RECOGNITION SITE==
<StructureSection load='1pvr' size='340' side='right'caption='[[1pvr]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pvr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PVR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvr OCA], [https://pdbe.org/1pvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pvr RCSB], [https://www.ebi.ac.uk/pdbsum/1pvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pvr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pvr ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures. The proteins share similar substitutions but differ in their preferences for the natural LoxP substrate and an engineered substrate that is inactive with wild-type Cre, LoxM7. One variant preferentially recombines LoxM7 and contacts the substituted bases through a hydrated network of novel interlocking protein-DNA contacts. The other variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone contact but different base contacts, facilitated by an unexpected DNA shift. Assisted by water, novel interaction networks can arise from few protein substitutions, suggesting how new DNA binding specificities might evolve. The contributions of macromolecular plasticity and water networks in specific DNA recognition observed here present a challenge for predictive schemes.


==Overview==
A specificity switch in selected cre recombinase variants is mediated by macromolecular plasticity and water.,Baldwin EP, Martin SS, Abel J, Gelato KA, Kim H, Schultz PG, Santoro SW Chem Biol. 2003 Nov;10(11):1085-94. PMID:14652076<ref>PMID:14652076</ref>
The basis for the altered DNA specificities of two Cre recombinase variants, obtained by mutation and selection, was revealed by their cocrystal structures. The proteins share similar substitutions but differ in their preferences for the natural LoxP substrate and an engineered substrate that is inactive with wild-type Cre, LoxM7. One variant preferentially recombines LoxM7 and contacts the substituted bases through a hydrated network of novel interlocking protein-DNA contacts. The other variant recognizes both LoxP and LoxM7 utilizing the same DNA backbone contact but different base contacts, facilitated by an unexpected DNA shift. Assisted by water, novel interaction networks can arise from few protein substitutions, suggesting how new DNA binding specificities might evolve. The contributions of macromolecular plasticity and water networks in specific DNA recognition observed here present a challenge for predictive schemes.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1PVR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p1 Enterobacteria phage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVR OCA].
</div>
<div class="pdbe-citations 1pvr" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
A specificity switch in selected cre recombinase variants is mediated by macromolecular plasticity and water., Baldwin EP, Martin SS, Abel J, Gelato KA, Kim H, Schultz PG, Santoro SW, Chem Biol. 2003 Nov;10(11):1085-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14652076 14652076]
*[[Resolvase 3D structures|Resolvase 3D structures]]
[[Category: Enterobacteria phage p1]]
== References ==
[[Category: Protein complex]]
<references/>
[[Category: Abel, J.]]
__TOC__
[[Category: Baldwin, E P.]]
</StructureSection>
[[Category: Gelato, K A.]]
[[Category: Escherichia virus P1]]
[[Category: Kim, H.]]
[[Category: Large Structures]]
[[Category: Martin, S S.]]
[[Category: Abel J]]
[[Category: Santoro, S W.]]
[[Category: Baldwin EP]]
[[Category: Schultz, P G.]]
[[Category: Gelato KA]]
[[Category: Cre]]
[[Category: Kim H]]
[[Category: Cre-loxp recombination]]
[[Category: Martin SS]]
[[Category: Dna]]
[[Category: Santoro SW]]
[[Category: Recombinase]]
[[Category: Schultz PG]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:32:32 2008''
Retrieved from "https://proteopedia.org/1pvr"