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[[Image:1q5z.jpg|left|200px]]


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==Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)==
The line below this paragraph, containing "STRUCTURE_1q5z", creates the "Structure Box" on the page.
<StructureSection load='1q5z' size='340' side='right'caption='[[1q5z]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1q5z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q5Z FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5z OCA], [https://pdbe.org/1q5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q5z RCSB], [https://www.ebi.ac.uk/pdbsum/1q5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q5z ProSAT]</span></td></tr>
{{STRUCTURE_1q5z|  PDB=1q5z  |  SCENE= }}
</table>
 
== Function ==
'''Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)'''
[https://www.uniprot.org/uniprot/SIPA_SALTY SIPA_SALTY] Actin-binding protein that interferes with host cell actin cytoskeleton. It stimulates actin polymerization and counteracts F-actin destabilizing proteins. Potentiates SipC activity; both are required for an efficient bacterial internalization. In vitro, forms a complex with host cell protein T-plastin increasing actin bundling. It inhibits ADF/cofilin-directed depolymerization both by preventing binding of ADF and cofilin and by displacing them from F-actin. Also protects F-actin from gelsolin-directed severing and reanneals gelsolin-severed F-actin fragments.<ref>PMID:10092234</ref> <ref>PMID:14992720</ref>
 
== References ==
 
<references/>
==Overview==
__TOC__
Like many bacterial pathogens, Salmonella spp. use a type III secretion system to inject virulence proteins into host cells. The Salmonella invasion protein A (SipA) binds host actin, enhances its polymerization near adherent extracellular bacteria, and contributes to cytoskeletal rearrangements that internalize the pathogen. By combining x-ray crystallography of SipA with electron microscopy and image analysis of SipA-actin filaments, we show that SipA functions as a "molecular staple," in which a globular domain and two nonglobular "arms" mechanically stabilize the filament by tethering actin subunits in opposing strands. Deletion analysis of the tethering arms provides strong support for this model.
</StructureSection>
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
1Q5Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Z OCA].  
[[Category: Egelman EH]]
 
[[Category: Galkin VE]]
==Reference==
[[Category: Lilic M]]
Salmonella SipA polymerizes actin by stapling filaments with nonglobular protein arms., Lilic M, Galkin VE, Orlova A, VanLoock MS, Egelman EH, Stebbins CE, Science. 2003 Sep 26;301(5641):1918-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14512630 14512630]
[[Category: Orlova A]]
[[Category: Salmonella typhimurium]]
[[Category: Stebbins CE]]
[[Category: Single protein]]
[[Category: VanLoock MS]]
[[Category: Egelman, E H.]]
[[Category: Galkin, V E.]]
[[Category: Lilic, M.]]
[[Category: Orlova, A.]]
[[Category: Stebbins, C E.]]
[[Category: VanLoock, M S.]]
[[Category: Cell invasion]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 05:54:26 2008''

Latest revision as of 08:13, 14 February 2024

Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)

1q5z, resolution 1.80Å

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