9f08: Difference between revisions
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New page: '''Unreleased structure''' The entry 9f08 is ON HOLD Authors: Ascham, A., Salihovic, A., Burley, G., Grogan, G. Description: Nucleoside-2''-deoxyribosyltransferase from Lactobacillus l... |
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==Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Covalent complex with 2-deoxyribose.== | |||
<StructureSection load='9f08' size='340' side='right'caption='[[9f08]], [[Resolution|resolution]] 2.37Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9f08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_leichmannii Lactobacillus leichmannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9F08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9F08 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.371Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DR:2-DEOXY-BETA-D-ERYTHRO-PENTOFURANOSE'>2DR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9f08 OCA], [https://pdbe.org/9f08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9f08 RCSB], [https://www.ebi.ac.uk/pdbsum/9f08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9f08 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NTD_LACLE NTD_LACLE] Catalyzes the cleavage of the glycosidic bond of 2'-deoxyribonucleosides and the transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base.<ref>PMID:7797550</ref> <ref>PMID:8805514</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nucleosides are pervasive building blocks that are found throughout nature and used extensively in medicinal chemistry and biotechnology. However, the preparation of base-modified analogues using conventional synthetic methodology poses challenges in scale-up and purification. In this work, an integrated approach involving structural analysis, screening and reaction optimization, is established to prepare 2'-deoxyribonucleoside analogues catalysed by the type II nucleoside 2'-deoxyribosyltransferase from Lactobacillus leichmannii (LlNDT-2). Structural analysis in combination with substrate profiling, identified the constraints on pyrimidine and purine acceptor bases by LlNDT2. A solvent screen identifies pure water as a suitable solvent for the preparation of high value purine and pyrimidine 2'-deoxyribonucleoside analogues on a gram scale under optimized reaction conditions. This approach provides the basis to establish a convergent, step-efficient chemoenzymatic platform for the preparation of high value 2'-deoxyribonucleosides. | |||
Gram-scale enzymatic synthesis of 2'-deoxyribonucleoside analogues using nucleoside transglycosylase-2.,Salihovic A, Ascham A, Taladriz-Sender A, Bryson S, Withers JM, McKean IJW, Hoskisson PA, Grogan G, Burley GA Chem Sci. 2024 Aug 27;15(37):15399-407. doi: 10.1039/d4sc04938a. PMID:39234214<ref>PMID:39234214</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9f08" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Salihovic | __TOC__ | ||
</StructureSection> | |||
[[Category: Lactobacillus leichmannii]] | |||
[[Category: Large Structures]] | |||
[[Category: Ascham A]] | |||
[[Category: Burley G]] | |||
[[Category: Grogan G]] | |||
[[Category: Salihovic A]] | |||
Latest revision as of 06:21, 4 December 2024
Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Covalent complex with 2-deoxyribose.
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