9f6v: Difference between revisions
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==cryoEM structure of Asgard tubulin heterodimer AtubA/B2 with GTP== | |||
<StructureSection load='9f6v' size='340' side='right'caption='[[9f6v]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9f6v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Lokiarchaeum_ossiferum Candidatus Lokiarchaeum ossiferum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9F6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9F6V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9f6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9f6v OCA], [https://pdbe.org/9f6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9f6v RCSB], [https://www.ebi.ac.uk/pdbsum/9f6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9f6v ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Microtubules are a hallmark of eukaryotes. Archaeal and bacterial homologs of tubulins typically form homopolymers and non-tubular superstructures. The origin of heterodimeric tubulins assembling into microtubules remains unclear. Here, we report the discovery of microtubule-forming tubulins in Asgard archaea, the closest known relatives of eukaryotes. These Asgard tubulins (AtubA/B) are closely related to eukaryotic alpha/beta-tubulins and the enigmatic bacterial tubulins BtubA/B. Proteomics of Candidatus Lokiarchaeum ossiferum showed that AtubA/B were highly expressed. Cryoelectron microscopy structures demonstrate that AtubA/B form eukaryote-like heterodimers, which assembled into 5-protofilament bona fide microtubules in vitro. The additional paralog AtubB2 lacks a nucleotide-binding site and competitively displaced AtubB. These AtubA/B2 heterodimers polymerized into 7-protofilament non-canonical microtubules. In a sub-population of Ca. Lokiarchaeum ossiferum cells, cryo-tomography revealed tubular structures, while expansion microscopy identified AtubA/B cytoskeletal assemblies. Our findings suggest a pre-eukaryotic origin of microtubules and provide a framework for understanding the fundamental principles of microtubule assembly. | |||
Microtubules in Asgard archaea.,Wollweber F, Xu J, Ponce-Toledo RI, Marxer F, Rodrigues-Oliveira T, Possnecker A, Luo ZH, Malit JJL, Kokhanovska A, Wieczorek M, Schleper C, Pilhofer M Cell. 2025 Mar 19:S0092-8674(25)00254-5. doi: 10.1016/j.cell.2025.02.027. PMID:40120574<ref>PMID:40120574</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9f6v" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Candidatus Lokiarchaeum ossiferum]] | |||
[[Category: Large Structures]] | |||
[[Category: Kokhanovska A]] | |||
[[Category: Malit JJL]] | |||
[[Category: Pilhofer M]] | |||
[[Category: Ponce-Toled RI]] | |||
[[Category: Rodrigues-Oliveira T]] | |||
[[Category: Schleper C]] | |||
[[Category: Wollweber F]] | |||
[[Category: Xu J]] | |||
