8zf1: Difference between revisions
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New page: '''Unreleased structure''' The entry 8zf1 is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal structure of a Chemo Triplet Photoenzyme (CTPe)== | |||
<StructureSection load='8zf1' size='340' side='right'caption='[[8zf1]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8zf1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris_MG1363 Lactococcus lactis subsp. cremoris MG1363]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZF1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U1O:N-(9-oxidanylidenethioxanthen-2-yl)ethanamide'>U1O</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zf1 OCA], [https://pdbe.org/8zf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zf1 RCSB], [https://www.ebi.ac.uk/pdbsum/8zf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zf1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A2RI36_LACLM A2RI36_LACLM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Artificial photoenzymes with novel catalytic modes not found in nature are in high demand; yet, they also present significant challenges in the field of biocatalysis. In this study, a chemogenetic modification strategy is developed to facilitate the rapid diversification of photoenzymes. This strategy integrates site-specific chemical conjugation of various artificial photosensitizers into natural protein cavities and the iterative mutagenesis in cell lysates. Through rounds of directed evolution, prominent visible-light-activatable photoenzyme variants were developed, featuring a thioxanthone chromophore. They successfully enabled the enantioselective [2 + 2] photocycloaddition of 2-carboxamide indoles, a class of UV-sensitive substrates that are traditionally challenging for known photoenzymes. Furthermore, the versatility of this photoenzyme is demonstrated in enantioselective whole-cell photobiocatalysis, enabling the efficient synthesis of enantioenriched cyclobutane-fused indoline tetracycles. These findings significantly expand the photophysical properties of artificial photoenzymes, a critical factor in enhancing their potential for harnessing excited-state reactivity in stereoselective transformations. | |||
Chemogenetic Evolution of Diversified Photoenzymes for Enantioselective [2 + 2] Cycloadditions in Whole Cells.,Guo J, Qian J, Cai D, Huang J, Yang X, Sun N, Zhang J, Pang T, Zhao W, Wu G, Chen X, Zhong F, Wu Y J Am Chem Soc. 2024 Jul 17;146(28):19030-19041. doi: 10.1021/jacs.4c03087. Epub , 2024 Jul 8. PMID:38976645<ref>PMID:38976645</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8zf1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactococcus lactis subsp. cremoris MG1363]] | |||
[[Category: Large Structures]] | |||
[[Category: Chen X]] | |||
[[Category: Guo J]] | |||
[[Category: Qian JY]] | |||
[[Category: Wu YZ]] | |||
[[Category: Zhong FR]] | |||
Latest revision as of 12:25, 17 July 2024
Crystal structure of a Chemo Triplet Photoenzyme (CTPe)
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