8yee: Difference between revisions
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==Cryo-EM structure of in vitro reconstituted Light-harvesting complex II trimer== | |||
<StructureSection load='8yee' size='340' side='right'caption='[[8yee]], [[Resolution|resolution]] 2.37Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8yee]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YEE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.37Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1LXP:(1~{R})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(1~{R},4~{R})-2,6,6-trimethyl-4-oxidanyl-cyclohex-2-en-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol'>A1LXP</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=NEX:(3S,3S,5R,5R,6R,6S,8R,9cis)-6,7-didehydro-5,5,6,6-tetrahydro-5,6-epoxy-beta,beta-carotene-3,3,5-triol'>NEX</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yee OCA], [https://pdbe.org/8yee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yee RCSB], [https://www.ebi.ac.uk/pdbsum/8yee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yee ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Light-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII protein must be identified. In vitro reconstitution with apoproteins synthesized by Escherichia coli and pigment-lipid mixtures from natural sources is an effective approach; however, the local environment surrounding each pigment within reconstituted LHCII (rLHCII) has only been indirectly estimated using spectroscopic and biochemical methods. Here, we used cryo-electron microscopy to determine the 3D structure of the rLHCII trimer and found that rLHCII exhibited a structure that was virtually identical to that of native LHCII, with a few exceptions: some C-terminal amino acids were not visible, likely due to aggregation of the His-tags; a carotenoid at the V1 site was not visible; and at site 614 showed mixed occupancy by both chlorophyll a and b molecules. Our observations confirmed the applicability of the in vitro reconstitution technique. | |||
Structure-based validation of recombinant light-harvesting complex II.,Seki S, Miyata T, Norioka N, Tanaka H, Kurisu G, Namba K, Fujii R PNAS Nexus. 2024 Sep 25;3(9):pgae405. doi: 10.1093/pnasnexus/pgae405. eCollection , 2024 Sep. PMID:39346626<ref>PMID:39346626</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8yee" style="background-color:#fffaf0;"></div> | ||
[[Category: Fujii | == References == | ||
[[Category: | <references/> | ||
[[Category: Miyata | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Seki | [[Category: Large Structures]] | ||
[[Category: Spinacia oleracea]] | |||
[[Category: Fujii R]] | |||
[[Category: Kurisu G]] | |||
[[Category: Miyata T]] | |||
[[Category: Namba K]] | |||
[[Category: Seki S]] | |||
[[Category: Tanaka H]] | |||
Latest revision as of 15:50, 13 August 2026
Cryo-EM structure of in vitro reconstituted Light-harvesting complex II trimer
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