9g49: Difference between revisions

Latest revision as of 09:07, 22 October 2025

Cryo-EM reconstruction of the full-length E. coli transmembrane formate transporter FocA

Structural highlights

9g49 is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.56Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FOCA_ECOLI Involved in the bidirectional transport of formate during mixed-acid fermentation (PubMed:23335413, PubMed:24659605, PubMed:30247527, PubMed:33169422, PubMed:35084298, PubMed:35377837, PubMed:35390794, PubMed:8022272). Functions to maintain relatively constant intracellular formate levels during growth, using different mechanisms for efflux and uptake of the anion (PubMed:35377837). Has a strong preference for formate as a substrate in vivo and not other acidic fermentation products (PubMed:23335413).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

References

↑ Beyer L, Doberenz C, Falke D, Hunger D, Suppmann B, Sawers RG. Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products. J Bacteriol. 2013 Apr;195(7):1428-35. PMID:23335413 doi:10.1128/JB.02166-12
↑ Hunger D, Doberenz C, Sawers RG. Identification of key residues in the formate channel FocA that control import and export of formate. Biol Chem. 2014 Jul;395(7-8):813-25. PMID:24659605 doi:10.1515/hsz-2014-0154
↑ Hakobyan B, Pinske C, Sawers G, Trchounian A, Trchounian K. pH and a mixed carbon-substrate spectrum influence FocA formate-driven H2 production in Escherichia coli. FEMS Microbiol Lett. 2018 Nov 1;365(21). PMID:30247527 doi:10.1093/femsle/fny233
↑ Kammel M, Hunger D, Sawers RG. The soluble cytoplasmic N-terminal domain of the FocA channel gates bidirectional formate translocation. Mol Microbiol. 2021 Apr;115(4):758-773. PMID:33169422 doi:10.1111/mmi.14641
↑ Kammel M, Trebbin O, Pinske C, Sawers RG. A single amino acid exchange converts FocA into a unidirectional efflux channel for formate. Microbiology (Reading). 2022 Jan;168(1):001132. PMID:35084298 doi:10.1099/mic.0.001132
↑ Kammel M, Sawers RG. The FocA channel functions to maintain intracellular formate homeostasis during Escherichia coli fermentation. Microbiology (Reading). 2022 Apr;168(4). PMID:35377837 doi:10.1099/mic.0.001168
↑ Kammel M, Trebbin O, Sawers RG. Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel. Microb Physiol. 2022;32(3-4):95-107. PMID:35390794 doi:10.1159/000524454
↑ Suppmann B, Sawers G. Isolation and characterization of hypophosphite--resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol. 1994 Mar;11(5):965-82. PMID:8022272 doi:10.1111/j.1365-2958.1994.tb00375.x

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OCA

[1] Beyer L, Doberenz C, Falke D, Hunger D, Suppmann B, Sawers RG. Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products. J Bacteriol. 2013 Apr;195(7):1428-35. PMID:23335413 doi:10.1128/JB.02166-12

[2] Hunger D, Doberenz C, Sawers RG. Identification of key residues in the formate channel FocA that control import and export of formate. Biol Chem. 2014 Jul;395(7-8):813-25. PMID:24659605 doi:10.1515/hsz-2014-0154

[3] Hakobyan B, Pinske C, Sawers G, Trchounian A, Trchounian K. pH and a mixed carbon-substrate spectrum influence FocA formate-driven H2 production in Escherichia coli. FEMS Microbiol Lett. 2018 Nov 1;365(21). PMID:30247527 doi:10.1093/femsle/fny233

[4] Kammel M, Hunger D, Sawers RG. The soluble cytoplasmic N-terminal domain of the FocA channel gates bidirectional formate translocation. Mol Microbiol. 2021 Apr;115(4):758-773. PMID:33169422 doi:10.1111/mmi.14641

[5] Kammel M, Trebbin O, Pinske C, Sawers RG. A single amino acid exchange converts FocA into a unidirectional efflux channel for formate. Microbiology (Reading). 2022 Jan;168(1):001132. PMID:35084298 doi:10.1099/mic.0.001132

[6] Kammel M, Sawers RG. The FocA channel functions to maintain intracellular formate homeostasis during Escherichia coli fermentation. Microbiology (Reading). 2022 Apr;168(4). PMID:35377837 doi:10.1099/mic.0.001168

[7] Kammel M, Trebbin O, Sawers RG. Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel. Microb Physiol. 2022;32(3-4):95-107. PMID:35390794 doi:10.1159/000524454

[8] Suppmann B, Sawers G. Isolation and characterization of hypophosphite--resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol. 1994 Mar;11(5):965-82. PMID:8022272 doi:10.1111/j.1365-2958.1994.tb00375.x

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9g49 is ON HOLD
+==Cryo-EM reconstruction of the full-length E. coli transmembrane formate transporter FocA==
+<StructureSection load='9g49' size='340' side='right'caption='[[9g49]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9g49]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9G49 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9G49 FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9g49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9g49 OCA], [https://pdbe.org/9g49 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9g49 RCSB], [https://www.ebi.ac.uk/pdbsum/9g49 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9g49 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FOCA_ECOLI FOCA_ECOLI] Involved in the bidirectional transport of formate during mixed-acid fermentation (PubMed:23335413, PubMed:24659605, PubMed:30247527, PubMed:33169422, PubMed:35084298, PubMed:35377837, PubMed:35390794, PubMed:8022272). Functions to maintain relatively constant intracellular formate levels during growth, using different mechanisms for efflux and uptake of the anion (PubMed:35377837). Has a strong preference for formate as a substrate in vivo and not other acidic fermentation products (PubMed:23335413).<ref>PMID:23335413</ref> <ref>PMID:24659605</ref> <ref>PMID:30247527</ref> <ref>PMID:33169422</ref> <ref>PMID:35084298</ref> <ref>PMID:35377837</ref> <ref>PMID:35390794</ref> <ref>PMID:8022272</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Hamdi F]]
+[[Category: Janson K]]
+[[Category: Kastritis PL]]
+[[Category: Kyrilis FL]]
+[[Category: Tueting C]]

9g49: Difference between revisions

Latest revision as of 09:07, 22 October 2025

Cryo-EM reconstruction of the full-length E. coli transmembrane formate transporter FocA

Structural highlights

Function

References

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