ConSurfDB vs. ConSurf: Difference between revisions
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[[FirstGlance in Jmol]] displays <scene name='39/399854/4dnw_consurf_apd-point48/2'>all salt bridges</scene> with one click (Tools tab), colored by conservation (if pre-processed by the ConSurf Server), and can list them, '''spreadsheet-ready, including conservation level numbers, and marking those between chains'''. | [[FirstGlance in Jmol]] displays <scene name='39/399854/4dnw_consurf_apd-point48/2'>all salt bridges</scene> with one click (Tools tab), colored by conservation (if pre-processed by the ConSurf Server), and can list them, '''spreadsheet-ready, including conservation level numbers, and marking those between chains'''. | ||
With the default ConSurf Server result '''APD 1.42''', and with a custom ConSurf Server result '''APD 0.91''', the salt-bridged residues have about '''average''' conservation. With a custom result '''APD 0.48''', the between-chain salt bridges have '''above-average''' conservation (7.6 vs. 6.8), while the within-chain salt bridges have below average conservation (6.3 vs. 6.8). In conclusion, when the multiple sequence alignment is limited to sequences closely related to the query (APD 0.48), '''between-chain salt bridged residues are more conserved than are within-chain salt bridged residues.''' The difference is '''statistically significant''' (p < 0.01<ref name="stats">With APD 0.48, mean conservation of between-chain salt bridged atoms is 7.57 ± 0.13 SEM. Subtracting 3 SEM (99% confidence limit) gives 7.18. This does not overlap with either 7.16 (the all-salt-bridged atoms mean + 3 SEM) or 6.82 (the mean for | With the default ConSurf Server result '''APD 1.42''', and with a custom ConSurf Server result '''APD 0.91''', the salt-bridged residues have about '''average''' conservation. With a custom result '''APD 0.48''', the between-chain salt bridges have '''above-average''' conservation (7.6 vs. 6.8), while the within-chain salt bridges have below average conservation (6.3 vs. 6.8). In conclusion, when the multiple sequence alignment is limited to sequences closely related to the query (APD 0.48), '''between-chain salt bridged residues are more conserved than are within-chain salt bridged residues.''' The difference is '''statistically significant''' (p < 0.01<ref name="stats">With APD 0.48, mean conservation of between-chain salt bridged atoms is 7.57 ± 0.13 SEM. Subtracting 3 SEM (99% confidence limit) gives 7.18. This does not overlap with either 7.16 (the all-salt-bridged atoms mean + 3 SEM) or 6.82 (the mean for within-chain salt-bridged atoms + 3 SEM).</ref>). | ||
<table class="wikitable" style="text-align:center;"> | <table class="wikitable" style="text-align:center;"> | ||
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18% | 18% | ||
</td><td> | </td><td> | ||
6.8 ± 0.12 | 6.8 ± 0.12* | ||
</td><td> | </td><td> | ||
7.6 ± 0.13 | 7.6 ± 0.13* | ||
</td><td> | </td><td> | ||
6.3 ± 0.17 | 6.3 ± 0.17* | ||
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
*Averages are per atom for | * *Averages are per atom for 88 between-chains salt-bridged atoms, and 140 within chain salt-bridged atoms. SEM's were calculated as standard deviation divided by the square root of the atom counts. Differences for APD 0.48 are statistically significant, p < 0.01<ref name="stats" />. | ||
*Salt bridges are Lys or Arg sidechain nitrogens within 4.0 Å of Asp or Glu sidechain oxygens. | *Salt bridges are Lys or Arg sidechain nitrogens within 4.0 Å of Asp or Glu sidechain oxygens. | ||
Examples of conserved patches on other proteins, revealed by ConSurf, will be found in the articles on | Examples of conserved patches on other proteins, revealed by ConSurf, will be found in the articles on | ||
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# Displays the protein, colored by conservation, in interactive 3D, using the NGL Viewer, [[FirstGlance in Jmol]], [[Chimera]], or [[PyMOL]]. | # Displays the protein, colored by conservation, in interactive 3D, using the NGL Viewer, [[FirstGlance in Jmol]], [[Chimera]], or [[PyMOL]]. | ||
==References== | ==See Also== | ||
*[[ConSurf/Index]] provides links to all pages about evolutionary conservation and ConSurf in Proteopedia. | |||
==Notes & References== | |||
{{Reflist}} | {{Reflist}} | ||
