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New page: left|200px<br /> <applet load="1wrd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wrd, resolution 1.75Å" /> '''Crystal structure o...
 
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[[Image:1wrd.gif|left|200px]]<br />
<applet load="1wrd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wrd, resolution 1.75&Aring;" />
'''Crystal structure of Tom1 GAT domain in complex with ubiquitin'''<br />


==Overview==
==Crystal structure of Tom1 GAT domain in complex with ubiquitin==
Tom1 (Target of Myb1) is suggested to be involved in the transport of, ubiquitinated proteins, through the interaction of its GAT (GGA and Tom1), domain with ubiquitin. Here, we demonstrate that the three-helix bundle of, Tom1-GAT has two ubiquitin-binding sites recognizing the hydrophobic Ile44, surface of ubiquitin. The complex crystal structure demonstrates that the, first site is a hydrophobic patch on helices alpha1 and alpha2. NMR and, biochemical data revealed that the N-terminal half of helix alpha3 of, Tom1-GAT constitutes the second, stronger binding site. The double-sided, ubiquitin binding enhances the efficiency of recognition of ubiquitinated, proteins by Tom1.
<StructureSection load='1wrd' size='340' side='right'caption='[[1wrd]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wrd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WRD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wrd OCA], [https://pdbe.org/1wrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wrd RCSB], [https://www.ebi.ac.uk/pdbsum/1wrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wrd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TOM1_HUMAN TOM1_HUMAN] May be involved in intracellular trafficking. Probable association with membranes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/1wrd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wrd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1WRD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WRD OCA].
*[[3D structures of ubiquitin|3D structures of ubiquitin]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domain., Akutsu M, Kawasaki M, Katoh Y, Shiba T, Yamaguchi Y, Kato R, Kato K, Nakayama K, Wakatsuki S, FEBS Lett. 2005 Oct 10;579(24):5385-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16199040 16199040]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Akutsu, M.]]
[[Category: Akutsu M]]
[[Category: Kato, K.]]
[[Category: Kato K]]
[[Category: Kato, R.]]
[[Category: Kato R]]
[[Category: Katoh, Y.]]
[[Category: Katoh Y]]
[[Category: Kawasaki, M.]]
[[Category: Kawasaki M]]
[[Category: Nakayama, K.]]
[[Category: Nakayama K]]
[[Category: Shiba, T.]]
[[Category: Shiba T]]
[[Category: Wakatsuki, S.]]
[[Category: Wakatsuki S]]
[[Category: Yamaguchi, Y.]]
[[Category: Yamaguchi Y]]
[[Category: three-helix bundle]]
[[Category: ubiquitin-binding protein]]
 
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Retrieved from "https://proteopedia.org/1wrd"