9d19: Difference between revisions
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==Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain== | |||
<StructureSection load='9d19' size='340' side='right'caption='[[9d19]], [[Resolution|resolution]] 2.88Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9d19]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9D19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9D19 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.88Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POV:(2S)-3-(HEXADECANOYLOXY)-2-[(9Z)-OCTADEC-9-ENOYLOXY]PROPYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>POV</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9d19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9d19 OCA], [https://pdbe.org/9d19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9d19 RCSB], [https://www.ebi.ac.uk/pdbsum/9d19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9d19 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/KCMA1_HUMAN KCMA1_HUMAN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
BK channels are large-conductance calcium (Ca(2+))-activated potassium channels crucial for neuronal excitability, muscle contraction, and neurotransmitter release. The pore-forming (alpha) subunits co-assemble with auxiliary (beta and gamma) subunits that modulate their function. Previous studies demonstrated that the N-termini of beta2-subunits can inactivate BK channels, but with no structural correlate. Here, we investigate BK beta2-subunit inactivation using cryo-electron microscopy, electrophysiology and molecular dynamics simulations. We find that the beta2 N-terminus occludes the pore only in the Ca(2+)-bound open state, via a ball-and-chain mechanism. The first three hydrophobic residues of beta2 are crucial for occlusion, while the remainder of the N-terminus remains flexible. Neither the closed channel conformation obtained in the absence of Ca(2+) nor an intermediate conformation found in the presence of Ca(2+) show density for the N-terminus of the beta2 subunit in their pore, likely due to narrower side access portals preventing their entry into the channel pore. | |||
Ball-and-chain inactivation of a human large conductance calcium-activated potassium channel.,Agarwal S, Kim ED, Lee S, Simon A, Accardi A, Nimigean CM Nat Commun. 2025 Feb 19;16(1):1769. doi: 10.1038/s41467-025-56844-4. PMID:39971906<ref>PMID:39971906</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Agarwal | <div class="pdbe-citations 9d19" style="background-color:#fffaf0;"></div> | ||
[[Category: Nimigean | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Agarwal S]] | |||
[[Category: Nimigean C]] | |||
Latest revision as of 08:11, 5 March 2025
Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain
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