2lzz

Solution structure of a mutant of the triheme cytochrome PpcA from Geobacter sulfurreducens sheds light on the role of the conserved aromatic residue F15

spinqualitylabelsall models 2lzz Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2lzz is a 1 chain structure with sequence from Geobacter sulfurreducens KN400. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Ligands: HEM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Extracellular electron transfer is one of the physiological hallmarks of Geobacteraceae. Most of the Geobacter species encode for more than 100 c-type cytochromes which are, in general, poorly conserved between individual species. An exception to this is the PpcA family of periplasmic triheme c-type cytochromes, which are the most abundant proteins in these bacteria. The functional characterization of PpcA showed that it has the necessary properties to couple e(-)/H(+) transfer, a fundamental step for ATP synthesis. The detailed thermodynamic characterization of a PpcA mutant, in which the strictly conserved residue phenylalanine 15 was replaced by leucine (PpcAF15L), showed that the global redox network of cooperativities among heme groups is altered, preventing the mutant from performing a concerted e(-)/H(+) transfer. In this work, we determined the solution structure of PpcAF15L mutant in the fully reduced state using NMR spectroscopy by producing (15)N-labeled protein. In addition, pH-dependent conformational changes were mapped onto the structure. The mutant structure obtained is well defined, with an average pairwise root-mean-square deviation of 0.36A for the backbone atoms and 1.14A for all heavy atoms. Comparison between the mutant and wild-type structures elucidated the contribution of phenylalanine 15 in the modulation of the functional properties of PpcA. Solution structure of a mutant of the triheme cytochrome PpcA from Geobacter sulfurreducens sheds light on the role of the conserved aromatic residue F15.,Dantas JM, Morgado L, Pokkuluri PR, Turner DL, Salgueiro CA Biochim Biophys Acta. 2013 Jan 8. pii: S0005-2728(12)01108-5. doi:, 10.1016/j.bbabio.2012.12.008. PMID:23313804 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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