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New page: left|200px<br /> <applet load="1xcr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xcr, resolution 1.70Å" /> '''Crystal Structure o...
 
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[[Image:1xcr.gif|left|200px]]<br />
<applet load="1xcr" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xcr, resolution 1.70&Aring;" />
'''Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold'''<br />


==Overview==
==Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold==
The human protein PTD012 is the longer product of an alternatively spliced, gene and was described to be localized in the nucleus. The X-ray structure, analysis at 1.7 A resolution of PTD012 through SAD phasing reveals a, monomeric protein and a novel fold. The shorter splice form was also, studied and appears to be unfolded and non-functional. The structure of, PTD012 displays an alphabetabetaalpha four-layer topology. A metal ion, residing between the central beta-sheets is partially coordinated by three, histidine residues. X-ray absorption near-edge structure (XANES) analysis, identifies the PTD012-bound ion as Zn(2+). Tetrahedral coordination of the, ion is completed by the carboxylate oxygen atom of an acetate molecule, taken up from the crystallization buffer. The binding of Zn(2+) to PTD012, is reminiscent of zinc-containing enzymes such as carboxypeptidase, carbonic anhydrase, and beta-lactamase. Biochemical assays failed to, demonstrate any of these enzyme activities in PTD012. However, PTD012, exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.
<StructureSection load='1xcr' size='340' side='right'caption='[[1xcr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1xcr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCR FirstGlance]. <br>
1XCR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcr OCA], [https://pdbe.org/1xcr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcr RCSB], [https://www.ebi.ac.uk/pdbsum/1xcr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcr ProSAT]</span></td></tr>
Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold., Manjasetty BA, Bussow K, Fieber-Erdmann M, Roske Y, Gobom J, Scheich C, Gotz F, Niesen FH, Heinemann U, Protein Sci. 2006 Apr;15(4):914-20. Epub 2006 Mar 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16522806 16522806]
</table>
== Function ==
[https://www.uniprot.org/uniprot/CK054_HUMAN CK054_HUMAN] Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.<ref>PMID:16522806</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcr ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Buessow, K.]]
[[Category: Buessow K]]
[[Category: Fieber-Erdmann, M.]]
[[Category: Fieber-Erdmann M]]
[[Category: Goetz, F.]]
[[Category: Goetz F]]
[[Category: Heinemann, U.]]
[[Category: Heinemann U]]
[[Category: Manjasetty, B.A.]]
[[Category: Manjasetty BA]]
[[Category: Roske, Y.]]
[[Category: Roske Y]]
[[Category: ACY]]
[[Category: ZN]]
[[Category: acetate buffer]]
[[Category: splice variant]]
[[Category: structural genomics]]
[[Category: zinc-containing fold]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:03:35 2007''

Latest revision as of 13:36, 13 March 2024

Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold

1xcr, resolution 1.70Å

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