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New page: left|200px<br /> <applet load="1xk5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xk5, resolution 2.4Å" /> '''Crystal structure of...
 
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[[Image:1xk5.gif|left|200px]]<br />
<applet load="1xk5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xk5, resolution 2.4&Aring;" />
'''Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide'''<br />


==Overview==
==Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide==
In higher eukaryotes the biogenesis of spliceosomal UsnRNPs involves a, nucleocytoplasmic shuttling cycle. After the m7G-cap-dependent export of, the snRNAs U1, U2, U4 and U5 to the cytoplasm, each of these snRNAs, associates with seven Sm proteins. Subsequently, the m7G-cap is, hypermethylated to the 2,2,7-trimethylguanosine (m3G)-cap. The import, adaptor snurportin1 recognises the m3G-cap and facilitates the nuclear, import of the UsnRNPs by binding to importin-beta. Here we report the, crystal structure of the m3G-cap-binding domain of snurportin1 with bound, m3GpppG at 2.4 A resolution, revealing a structural similarity to the, mRNA-guanyly-transferase. Snurportin1 binds both the hypermethylated cap, and the first nucleotide of the RNA in a stacked conformation. This, binding mode differs significantly from that of the m7G-cap-binding, proteins Cap-binding protein 20 (CBP20), eukaryotic initiation factor 4E, (eIF4E) and viral protein 39 (VP39). The specificity of the m3G-cap, recognition by snurportin1 was evaluated by fluorescence spectroscopy, demonstrating the importance of a highly solvent exposed tryptophan for, the discrimination of m7G-capped RNAs. The critical role of this, tryptophan and as well of a tryptophan continuing the RNA base stack was, confirmed by nuclear import assays and cap-binding activity tests using, several snurportin1 mutants.
<StructureSection load='1xk5' size='340' side='right'caption='[[1xk5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1xk5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XK5 FirstGlance]. <br>
1XK5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with TPG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XK5 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPG:2,2,7-TRIMETHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>TPG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xk5 OCA], [https://pdbe.org/1xk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xk5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xk5 ProSAT]</span></td></tr>
Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1., Strasser A, Dickmanns A, Luhrmann R, Ficner R, EMBO J. 2005 Jul 6;24(13):2235-43. Epub 2005 May 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15920472 15920472]
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPN1_HUMAN SPN1_HUMAN] Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs.<ref>PMID:9670026</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xk/1xk5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xk5 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dickmanns, A.]]
[[Category: Dickmanns A]]
[[Category: Ficner, R.]]
[[Category: Ficner R]]
[[Category: Luehrmann, R.]]
[[Category: Luehrmann R]]
[[Category: Strasser, A.]]
[[Category: Strasser A]]
[[Category: TPG]]
[[Category: protein-rna-complex]]
 
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Retrieved from "https://proteopedia.org/1xk5"