9dsm: Difference between revisions
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==Cryo-EM structure of SSNA-1(R18E/R20E/Q98E) filaments== | |||
<StructureSection load='9dsm' size='340' side='right'caption='[[9dsm]], [[Resolution|resolution]] 4.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9dsm]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DSM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.55Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dsm OCA], [https://pdbe.org/9dsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dsm RCSB], [https://www.ebi.ac.uk/pdbsum/9dsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dsm ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5F4U5_CAEEL Q5F4U5_CAEEL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SSNA1 is a fibrillar protein involved in dynamic microtubule remodeling, including nucleation, co-polymerization, and microtubule branching. The underlying molecular mechanism has remained unclear due to a lack of structural information. Here, we determine the cryo-EM structure of C.elegans SSNA-1 at 4.55-A resolution and evaluate its role in embryonic development. We find that SSNA-1 forms an anti-parallel coiled-coil, with self-assembly facilitated by an overhang of 16 C-terminal residues that form a triple-stranded helical junction. The microtubule-binding region is within the triple-stranded junction, suggesting that self-assembly of SSNA-1 creates hubs for effective microtubule interaction. Genetical analysis elucidates that SSNA-1 deletion significantly reduces embryonic viability, and causes multipolar spindles during cell division. Interestingly, impairing SSNA-1 self-assembly has a comparable effect on embryonic viability as the knockout strain. Our study provides molecular insights into SSNA-1's self-assembly and its role in microtubule binding and cell division regulation through centriole stability. | |||
Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance.,Agostini L, Pfister JA, Basnet N, Ding J, Zhang R, Biertumpfel C, O'Connell KF, Mizuno N Nat Commun. 2025 Aug 13;16(1):7512. doi: 10.1038/s41467-025-62696-9. PMID:40804232<ref>PMID:40804232</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9dsm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Caenorhabditis elegans]] | |||
[[Category: Large Structures]] | |||
[[Category: Agostini L]] | |||
[[Category: Biertumpfel C]] | |||
[[Category: Mizuno N]] | |||
Latest revision as of 07:09, 27 August 2025
Cryo-EM structure of SSNA-1(R18E/R20E/Q98E) filaments
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