1u3e: Difference between revisions

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[[Image:1u3e.gif|left|200px]]


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==DNA binding and cleavage by the HNH homing endonuclease I-HmuI==
The line below this paragraph, containing "STRUCTURE_1u3e", creates the "Structure Box" on the page.
<StructureSection load='1u3e' size='340' side='right'caption='[[1u3e]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1u3e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_SPO1 Bacillus virus SPO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U3E FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
{{STRUCTURE_1u3e| PDB=1u3e |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u3e OCA], [https://pdbe.org/1u3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u3e RCSB], [https://www.ebi.ac.uk/pdbsum/1u3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u3e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HMUI_BPSP1 HMUI_BPSP1]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u3e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u3e ConSurf].
<div style="clear:both"></div>


'''DNA binding and cleavage by the HNH homing endonuclease I-HmuI'''
==See Also==
 
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
[[Category: Bacillus virus SPO1]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Landthaler M]]
1U3E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spo1 Bacillus phage spo1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA].
[[Category: Shen BW]]
 
[[Category: Shub DA]]
==Reference==
[[Category: Stoddard BL]]
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313606 15313606]
[[Category: Bacillus phage spo1]]
[[Category: Single protein]]
[[Category: Landthaler, M.]]
[[Category: Shen, B W.]]
[[Category: Shub, D A.]]
[[Category: Stoddard, B L.]]
[[Category: Helix-turn-helix dna binding domain]]
[[Category: Hnh catalytic motif]]
[[Category: Protein-dna complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:42:44 2008''

Latest revision as of 08:43, 14 February 2024

DNA binding and cleavage by the HNH homing endonuclease I-HmuI

1u3e, resolution 2.92Å

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