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[[Image:1u55.gif|left|200px]]
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{{STRUCTURE_1u55|  PDB=1u55  |  SCENE=  }}
'''Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)'''


==Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)==
<StructureSection load='1u55' size='340' side='right'caption='[[1u55]], [[Resolution|resolution]] 1.77&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1u55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U55 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u55 OCA], [https://pdbe.org/1u55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u55 RCSB], [https://www.ebi.ac.uk/pdbsum/1u55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u55 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u55_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u55 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Soluble guanylate cyclases are nitric oxide-responsive signaling proteins in which the nitric oxide sensor is a heme-binding domain of unknown structure that we have termed the heme-NO and oxygen binding (H-NOX) domain. H-NOX domains are also found in bacteria, either as isolated domains, or are fused through a membrane-spanning region to methyl-accepting chemotaxis proteins. We have determined the crystal structure of an oxygen-binding H-NOX domain of one such signaling protein from the obligate anaerobe Thermoanaerobacter tengcongensis at 1.77-angstroms resolution, revealing a protein fold unrelated to known structures. Particularly striking is the structure of the protoporphyrin IX group, which is distorted from planarity to an extent not seen before in protein-bound heme groups. Comparison of the structure of the H-NOX domain in two different crystal forms suggests a mechanism whereby alteration in the degree of distortion of the heme group is coupled to changes on the molecular surface of the H-NOX domain and potentially to changes in intermolecular interactions.


==Overview==
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.,Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12854-9. Epub 2004 Aug 23. PMID:15326296<ref>PMID:15326296</ref>
Soluble guanylate cyclases are nitric oxide-responsive signaling proteins in which the nitric oxide sensor is a heme-binding domain of unknown structure that we have termed the heme-NO and oxygen binding (H-NOX) domain. H-NOX domains are also found in bacteria, either as isolated domains, or are fused through a membrane-spanning region to methyl-accepting chemotaxis proteins. We have determined the crystal structure of an oxygen-binding H-NOX domain of one such signaling protein from the obligate anaerobe Thermoanaerobacter tengcongensis at 1.77-angstroms resolution, revealing a protein fold unrelated to known structures. Particularly striking is the structure of the protoporphyrin IX group, which is distorted from planarity to an extent not seen before in protein-bound heme groups. Comparison of the structure of the H-NOX domain in two different crystal forms suggests a mechanism whereby alteration in the degree of distortion of the heme group is coupled to changes on the molecular surface of the H-NOX domain and potentially to changes in intermolecular interactions.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1U55 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U55 OCA].
</div>
<div class="pdbe-citations 1u55" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases., Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J, Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12854-9. Epub 2004 Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15326296 15326296]
*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
[[Category: Single protein]]
*[[Methyl-accepting chemotaxis protein|Methyl-accepting chemotaxis protein]]
[[Category: Thermoanaerobacter tengcongensis]]
== References ==
[[Category: Boon, E M.]]
<references/>
[[Category: Karow, D S.]]
__TOC__
[[Category: Kuriyan, J.]]
</StructureSection>
[[Category: Marletta, M A.]]
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
[[Category: Pellicena, P.]]
[[Category: Large Structures]]
[[Category: Chemotaxis]]
[[Category: Boon EM]]
[[Category: H-nox domain]]
[[Category: Karow DS]]
[[Category: Heme]]
[[Category: Kuriyan J]]
[[Category: Oxygen sensor]]
[[Category: Marletta MA]]
[[Category: Signal transduction]]
[[Category: Pellicena P]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 10:45:59 2008''

Latest revision as of 07:15, 13 August 2026

Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)

1u55, resolution 1.77Å

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