9hrm: Difference between revisions
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New page: '''Unreleased structure''' The entry 9hrm is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal Structure of GH19 domain of D29-LysA (Form III)== | |||
<StructureSection load='9hrm' size='340' side='right'caption='[[9hrm]], [[Resolution|resolution]] 2.04Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9hrm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_phage_D29 Mycobacterium phage D29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HRM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hrm OCA], [https://pdbe.org/9hrm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hrm RCSB], [https://www.ebi.ac.uk/pdbsum/9hrm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hrm ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mycobacterial infections, including tuberculosis, remain a major global health challenge, causing millions of deaths annually. Their treatment is increasingly hindered by limited therapeutic options and rising antimicrobial resistance, highlighting the urgent need for alternative strategies. Mycobacteriophage LysA endolysins are complex multi-domain peptidoglycan hydrolases emerging as potential tools to treat mycobacterial infections. However, despite the therapeutic prospects of LysAs, our understanding of their mechanism of action remains limited. This study provides a comprehensive structural-functional analysis of the catalytic domains of D29LysA and DS6ALysA endolysins (D29N4/D29GH19 and DS6AGH19/DS6AAmi2B), characterised alone and in complex with PG analogues, using protein engineering, X-ray crystallography, small-angle X-ray scattering, and in silico tools. Our results reveal precise details of the substrate-binding site and the catalytic platforms at each domain, including information about substrate-binding mode and conformational changes associated with peptidoglycan recognition and hydrolysis. Moreover, these findings also suggest a coordinated mechanism of action of both catalytic domains in DS6ALysA lysin. These insights represent a significant advance in understanding the structural basis of mycobacterial cell-wall degradation by mycobacteriophage endolysins. Information that may aid in further exploring these endolysins as therapeutic antimicrobial tools in the future. | |||
Dissecting the molecular basis underlying mycobacterial cell-wall hydrolysis by the catalytic domains of D29LysA and DS6ALysA phage endolysins.,Ceballos-Zuniga F, Galvez-Larrosa L, Munoz IG, Infantes L, Fernandez-Carrillo J, Perez-Dorado I Int J Biol Macromol. 2025 Dec;334(Pt 1):148896. doi: , 10.1016/j.ijbiomac.2025.148896. Epub 2025 Nov 7. PMID:41207583<ref>PMID:41207583</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9hrm" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycobacterium phage D29]] | |||
[[Category: Ceballos-Zuniga F]] | |||
[[Category: Galvez-Larrosa L]] | |||
[[Category: Perez-Dorado I]] | |||
