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[[Image:1uup.gif|left|200px]]
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{{STRUCTURE_1uup|  PDB=1uup  |  SCENE=  }}
'''CRYSTAL STRUCTURE OF A DIMERIC FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN A (SPEA1).'''


==Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).==
<StructureSection load='1uup' size='340' side='right'caption='[[1uup]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1uup]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UUP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uup OCA], [https://pdbe.org/1uup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uup RCSB], [https://www.ebi.ac.uk/pdbsum/1uup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uup ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPEA_STRPY SPEA_STRPY] Causative agent of the symptoms associated with scarlet fever, have been associated with streptococcal toxic shock-like disease and may play a role in the early events of rheumatic fever.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uup_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uup ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen associated with scarlet fever and streptococcal toxic shock syndrome (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous work suggested that the dimer results from an intermolecular disulfide bond between the cysteines at positions 90 of each monomer. Here, we present the crystal structure of the dimeric form of SpeA1. The toxin crystallizes in the orthorhombic space group P212121, with two dimers in the crystallographic asymmetric unit. The final structure has a crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water molecules, and 4 zinc atoms (one zinc atom per molecule). The implications of SpeA1 dimer on MHC class II and T-cell receptor recognition are discussed.


==Overview==
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1).,Baker MD, Gendlina I, Collins CM, Acharya KR Protein Sci. 2004 Sep;13(9):2285-90. Epub 2004 Aug 4. PMID:15295110<ref>PMID:15295110</ref>
Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen associated with scarlet fever and streptococcal toxic shock syndrome (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous work suggested that the dimer results from an intermolecular disulfide bond between the cysteines at positions 90 of each monomer. Here, we present the crystal structure of the dimeric form of SpeA1. The toxin crystallizes in the orthorhombic space group P212121, with two dimers in the crystallographic asymmetric unit. The final structure has a crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water molecules, and 4 zinc atoms (one zinc atom per molecule). The implications of SpeA1 dimer on MHC class II and T-cell receptor recognition are discussed.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1UUP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUP OCA].
</div>
<div class="pdbe-citations 1uup" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1)., Baker MD, Gendlina I, Collins CM, Acharya KR, Protein Sci. 2004 Sep;13(9):2285-90. Epub 2004 Aug 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15295110 15295110]
*[[Exotoxin 3D structures|Exotoxin 3D structures]]
[[Category: Single protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pyogenes]]
[[Category: Streptococcus pyogenes]]
[[Category: Acharya, K R.]]
[[Category: Acharya KR]]
[[Category: Baker, M D.]]
[[Category: Baker MD]]
[[Category: Collins, C M.]]
[[Category: Collins CM]]
[[Category: Gendlina, I.]]
[[Category: Gendlina I]]
[[Category: Dimer]]
[[Category: Exotoxin]]
[[Category: Molecular recognition]]
[[Category: Superantigen]]
[[Category: Toxin]]
[[Category: X-ray crystallography]]
[[Category: Zinc binding]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 11:43:03 2008''
Retrieved from "https://proteopedia.org/1uup"