9n1c: Difference between revisions
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New page: '''Unreleased structure''' The entry 9n1c is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==Crystal structure of CysS from Corallococcus sp. CA054B with 5'-deoxyadenosine, methionine, and pantetheinylated 3-ethoxy-4-amino benzoic acid substrate bound== | ||
<StructureSection load='9n1c' size='340' side='right'caption='[[9n1c]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9n1c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corallococcus_sp._CA054B Corallococcus sp. CA054B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9N1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9N1C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=A1BUP:2-({N-[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]-beta-alanyl}amino)ethyl+4-amino-3-ethoxybenzoate'>A1BUP</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9n1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9n1c OCA], [https://pdbe.org/9n1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9n1c RCSB], [https://www.ebi.ac.uk/pdbsum/9n1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9n1c ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cystobactamids are nonribosomal peptide natural products that function as DNA gyrase inhibitors, exhibiting significant antibacterial activity. They are isolated from Cystobacter sp. Cbv34 and contain various alkoxy groups on para-aminobenzoic acid moieties, which are believed to play a crucial role in antibacterial functions. The alkoxy groups are generated by iterative methylations on a methoxy group by the cobalamin (Cbl)-dependent radical S-adenosylmethionine (SAM) enzyme CysS. CysS catalyzes up to three methylations to give ethoxy, isopropoxy, sec-butoxy, and tert-butoxy groups. For each methylation, CysS uses a ping-pong mechanism in which two molecules of SAM are consumed. One SAM is used to methylate cob(I)alamin, while another generates a 5'-deoxyadenosyl 5'-radical to initiate substrate methylation. However, little is known about how the enzyme promotes both Cbl methylation and iterative substrate methylation, which occur by polar S(N)2 and radical processes, respectively. Here, we report three X-ray crystal structures of a homolog of CysS from Corallococcus sp. CA054B. Two were determined in the presence of methoxy- and ethoxy-containing substrates, showing how CysS accommodates substrates and products during iterative methylation. The third structure, determined in the absence of a substrate, exhibits structural changes that reorient the SAM's conformation to allow for the methylation of cob(I)alamin. | |||
Structural basis for iterative methylation by a cobalamin-dependent radical S-adenosylmethionine enzyme in cystobactamids biosynthesis.,Cui J, Wang B, Maurya RK, Booker SJ Proc Natl Acad Sci U S A. 2026 Jan 27;123(4):e2527019123. doi: , 10.1073/pnas.2527019123. Epub 2026 Jan 21. PMID:41564129<ref>PMID:41564129</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9n1c" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Corallococcus sp. CA054B]] | |||
[[Category: Large Structures]] | |||
[[Category: Booker SJ]] | |||
[[Category: Cui J]] | |||
[[Category: Wang B]] | |||
Latest revision as of 14:36, 10 February 2026
Crystal structure of CysS from Corallococcus sp. CA054B with 5'-deoxyadenosine, methionine, and pantetheinylated 3-ethoxy-4-amino benzoic acid substrate bound
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