9ujs: Difference between revisions
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New page: '''Unreleased structure''' The entry 9ujs is ON HOLD Authors: Description: Category: Unreleased Structures |
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The | ==RNA polymerase II elongation complex stalled at SHL(-4) of the H3-H4 octasome== | ||
<StructureSection load='9ujs' size='340' side='right'caption='[[9ujs]], [[Resolution|resolution]] 3.62Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9ujs]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii_GS115 Komagataella phaffii GS115]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9UJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9UJS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.62Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ujs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ujs OCA], [https://pdbe.org/9ujs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ujs RCSB], [https://www.ebi.ac.uk/pdbsum/9ujs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ujs ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/C4R4Y0_KOMPG C4R4Y0_KOMPG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The histone H3-H4 octasome is a nucleosome-like particle in which two DNA gyres are wrapped around each histone (H3-H4)(2) tetramer disk, forming a clamshell-like configuration. In the present study, we performed in vitro RNA polymerase II (RNAPII) transcription assays with the H3-H4 octasome and found that RNAPII transcribed the H3-H4 octasome more efficiently than the nucleosome. RNAPII paused at only one position, superhelical location (SHL(-4)) in the H3-H4 octasome, in contrast to pausing at the SHL(-5), SHL(-2), and SHL(-1) positions in the nucleosome. Cryo-EM analysis revealed that two (H3-H4)(2) tetramer disks are retained when the RNAPII paused at the SHL(-4) position of the H3-H4 octasome. However, when RNAPII reached the SHL(-0.5) position, five base pairs before the dyad position of the H3-H4 octasome, the proximal (H3-H4)(2) tetramer was disassembled, but the distal (H3-H4)(2) tetramer still remained on the DNA. Therefore, RNAPII efficiently transcribes the H3-H4 octasome by stepwise (H3-H4)(2) tetramer disassembly. | |||
Structural basis of RNA polymerase II transcription on the histone H3-H4 octasome.,Ho CH, Nozawa K, Nishimura M, Oi M, Kujirai T, Ogasawara M, Ehara H, Sekine SI, Takizawa Y, Kurumizaka H J Biol Chem. 2026 Mar 4;302(4):111340. doi: 10.1016/j.jbc.2026.111340. PMID:41791711<ref>PMID:41791711</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9ujs" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Komagataella phaffii GS115]] | |||
[[Category: Large Structures]] | |||
[[Category: Ehara H]] | |||
[[Category: Ho C-H]] | |||
[[Category: Kujirai T]] | |||
[[Category: Kurumizaka H]] | |||
[[Category: Nishimura M]] | |||
[[Category: Nozawa K]] | |||
[[Category: Ogasawara M]] | |||
[[Category: Oi M]] | |||
[[Category: Sekine S]] | |||
[[Category: Takizawa Y]] | |||
Latest revision as of 09:33, 15 April 2026
RNA polymerase II elongation complex stalled at SHL(-4) of the H3-H4 octasome
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