Sandbox 326: Difference between revisions

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'''Research Question:''' What is the function of the 3B7F protein, and how can this be determined through both computational and wet lab techniques?

'''Relevance:''' The goal of this research is to determine the function of the 3BF7 protein in order to evaluate whether it can degrade xyloglucan or other types of carbohydrates and glycoconjugates in plants. Knowing this will allow future researchers to be able to better understand xyloglucan/carbohydrate glycoconjugate degradation in plants, and allow for known pathways to be expanded upon. Through this experimentation, we can also learn more about the ways in which both computational bioinformatics and wet lab techniques can aid in determining the function of a protein with a known structure.

'''Relevance:''' The goal of this research is to determine the function of the 3BF7 protein in order to evaluate whether it can degrade xyloglucan or other types of carbohydrates and glycoconjugates in plants. Knowing this will allow future researchers to be able to better understand xyloglucan/carbohydrate/glycoconjugate degradation in plants, and allow for known pathways to be expanded upon. Through this experimentation, we can also learn more about the ways in which both computational bioinformatics and wet lab techniques can aid in determining the function of a protein with a known structure.

'''Hypothesis:''' 3B7F is a xyloglucanase, a type of glycosyl hydrolase that acts to degrade xyloglucan in plant cell walls. It presents optimal activity in fairly acidic conditions and demonstrates potentially satisfactory binding with PNP phosphate and lysine p nitroanilide.

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[6] SwissDock. www.swissdock.ch. https://www.swissdock.ch/.

[7]Shi, H.; Guo, J.; Yan, X.; Cui, G.; Tan, Z.; Zhu, X.; Zhou, J.; He, S.; Wang, T.; Li, X. Characterization of a Xyloglucananse in Biodegradation of Woody Plant Xyloglucan from Caldicellulosiruptor Kronotskyensis. BioResources 2021, 17 (1), 673–681. https://doi.org/10.15376/biores.17.1.673-681.

[7] Shi, H.; Guo, J.; Yan, X.; Cui, G.; Tan, Z.; Zhu, X.; Zhou, J.; He, S.; Wang, T.; Li, X. Characterization of a Xyloglucananse in Biodegradation of Woody Plant Xyloglucan from Caldicellulosiruptor Kronotskyensis. BioResources 2021, 17 (1), 673–681. https://doi.org/10.15376/biores.17.1.673-681.

[8] Doggwiler, V.; Lanz, M.; Paredes, V.; Lipps, G.; Imanidis, G. Tablet Formulation with Dual Control Concept for Efficient Colonic Drug Delivery. International Journal of Pharmaceutics 2023, 631, 122499. https://doi.org/10.1016/j.ijpharm.2022.122499.

Sandbox 326: Difference between revisions

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@@ Line 6: / Line 6: @@
 '''Research Question:''' What is the function of the 3B7F protein, and how can this be determined through both computational and wet lab techniques?
-'''Relevance:''' The goal of this research is to determine the function of the 3BF7 protein in order to evaluate whether it can degrade xyloglucan or other types of carbohydrates and glycoconjugates in plants. Knowing this will allow future researchers to be able to better understand xyloglucan/carbohydrate glycoconjugate degradation in plants, and allow for known pathways to be expanded upon. Through this experimentation, we can also learn more about the ways in which both computational bioinformatics and wet lab techniques can aid in determining the function of a protein with a known structure.
+'''Relevance:''' The goal of this research is to determine the function of the 3BF7 protein in order to evaluate whether it can degrade xyloglucan or other types of carbohydrates and glycoconjugates in plants. Knowing this will allow future researchers to be able to better understand xyloglucan/carbohydrate/glycoconjugate degradation in plants, and allow for known pathways to be expanded upon. Through this experimentation, we can also learn more about the ways in which both computational bioinformatics and wet lab techniques can aid in determining the function of a protein with a known structure.
 '''Hypothesis:''' 3B7F is a xyloglucanase, a type of glycosyl hydrolase that acts to degrade xyloglucan in plant cell walls. It presents optimal activity in fairly acidic conditions and demonstrates potentially satisfactory binding with PNP phosphate and lysine p nitroanilide.
@@ Line 152: / Line 152: @@
 [6] SwissDock. www.swissdock.ch. https://www.swissdock.ch/.
-[7]Shi, H.; Guo, J.; Yan, X.; Cui, G.; Tan, Z.; Zhu, X.; Zhou, J.; He, S.; Wang, T.; Li, X. Characterization of a Xyloglucananse in Biodegradation of Woody Plant Xyloglucan from Caldicellulosiruptor Kronotskyensis. BioResources 2021, 17 (1), 673–681. https://doi.org/10.15376/biores.17.1.673-681.
+[7] Shi, H.; Guo, J.; Yan, X.; Cui, G.; Tan, Z.; Zhu, X.; Zhou, J.; He, S.; Wang, T.; Li, X. Characterization of a Xyloglucananse in Biodegradation of Woody Plant Xyloglucan from Caldicellulosiruptor Kronotskyensis. BioResources 2021, 17 (1), 673–681. https://doi.org/10.15376/biores.17.1.673-681.
 [8] Doggwiler, V.; Lanz, M.; Paredes, V.; Lipps, G.; Imanidis, G. Tablet Formulation with Dual Control Concept for Efficient Colonic Drug Delivery. International Journal of Pharmaceutics 2023, 631, 122499. https://doi.org/10.1016/j.ijpharm.2022.122499.