9ulp: Difference between revisions
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New page: '''Unreleased structure''' The entry 9ulp is ON HOLD Authors: Fernandez-Perez, J., Caaveiro, J.M.M., Tsumoto, K. Description: Crystal structure of FtsB from Streptococcus pyogenes in c... |
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The | ==Crystal structure of FtsB from Streptococcus pyogenes in complex with Nb1 nanobody== | ||
<StructureSection load='9ulp' size='340' side='right'caption='[[9ulp]], [[Resolution|resolution]] 2.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9ulp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ULP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ULP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ulp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ulp OCA], [https://pdbe.org/9ulp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ulp RCSB], [https://www.ebi.ac.uk/pdbsum/9ulp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ulp ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A5S4TPK8_STRPY A0A5S4TPK8_STRPY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Due to the limited availability of metals inside the human body, pathogenic bacteria must produce multiple highly specialized metal transporters to cause infection. These transporters constitute attractive targets for developing novel antibacterial strategies. Streptococcus pyogenes possesses three iron transporters, of which the FtsABCD system is specialized in the uptake of ferric hydroxamates. The role of this transporter in infection remains unclear. In this study, we developed a monoclonal alpaca VHH, or nanobody, Nb1, targeting FtsB. Nb1 binds to FtsB with sub-nM affinity, in an enthalpy-driven manner, and with a characteristically slow dissociation rate. Solvent accessibility analysis by hydrogen/deuterium exchange coupled with mass spectrometry, mutational analyses, and X-ray crystallography revealed that the epitope of Nb1 is in the binding pocket of FtsB. The nanobody competitively inhibited the binding of multiple hydroxamate siderophores and partially inhibited the uptake of siderophores in S. pyogenes cells. The inhibitory activity of Nb1 on siderophore transport represents a new tool to study the role of the FtsABCD transporter and can be used as a potential inhibitor of S. pyogenes growth under iron-limited conditions. | |||
Development of an inhibitory monoclonal nanobody targeting Streptococcus pyogenes siderophore binding protein FtsB.,Fernandez-Perez J, de Vega S, Caaveiro JMM, Nakakido M, Nagatoishi S, Senoo A, Tanoi K, Nozawa T, Nakagawa I, Tsumoto K J Biol Chem. 2026 Feb 4;302(3):111224. doi: 10.1016/j.jbc.2026.111224. PMID:41651419<ref>PMID:41651419</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9ulp" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Caaveiro | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus pyogenes]] | |||
[[Category: Vicugna pacos]] | |||
[[Category: Caaveiro JMM]] | |||
[[Category: Fernandez-Perez J]] | |||
[[Category: Tsumoto K]] | |||
