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[[Image:1vrq.gif|left|200px]]
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{{STRUCTURE_1vrq|  PDB=1vrq  |  SCENE=  }}
'''Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid'''


==Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid==
<StructureSection load='1vrq' size='340' side='right'caption='[[1vrq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1vrq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_sp._U-96 Corynebacterium sp. U-96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VRQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMG:N,N-DIMETHYLGLYCINE'>DMG</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=FON:N-{[4-({[(6R)-2-AMINO-5-FORMYL-4-OXO-1,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)PHENYL]CARBONYL}-L-GLUTAMIC+ACID'>FON</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vrq OCA], [https://pdbe.org/1vrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vrq RCSB], [https://www.ebi.ac.uk/pdbsum/1vrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vrq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q50LF1_9CORY Q50LF1_9CORY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vr/1vrq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vrq ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The alpha subunit is composed of two domains, contains NAD(+), and binds folinic acid. The beta subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10A apart. The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure. The delta subunit contains a single atom of zinc and has a Cys(3)His zinc finger structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine oxidase is discussed.


==Overview==
Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96.,Ida K, Moriguchi T, Suzuki H Biochem Biophys Res Commun. 2005 Jul 29;333(2):359-66. PMID:15946648<ref>PMID:15946648</ref>
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme. Here we report the crystal structures of the enzyme in complex with dimethylglycine and folinic acid. The alpha subunit is composed of two domains, contains NAD(+), and binds folinic acid. The beta subunit contains dimethylglycine, FAD, and FMN, and these flavins are approximately 10A apart. The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure. The delta subunit contains a single atom of zinc and has a Cys(3)His zinc finger structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine oxidase is discussed.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1VRQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRQ OCA].
</div>
<div class="pdbe-citations 1vrq" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96., Ida K, Moriguchi T, Suzuki H, Biochem Biophys Res Commun. 2005 Jul 29;333(2):359-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15946648 15946648]
*[[Sarcosine oxidase|Sarcosine oxidase]]
[[Category: Corynebacterium sp.]]
== References ==
[[Category: Protein complex]]
<references/>
[[Category: Sarcosine oxidase]]
__TOC__
[[Category: Ida, K.]]
</StructureSection>
[[Category: Moriguchi, T.]]
[[Category: Corynebacterium sp. U-96]]
[[Category: Suzuki, H.]]
[[Category: Large Structures]]
[[Category: Flavoenzyme]]
[[Category: Ida K]]
[[Category: Heterotetrameric sarcosine oxidase]]
[[Category: Moriguchi T]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 12:50:38 2008''
[[Category: Suzuki H]]

Latest revision as of 00:05, 28 December 2023

Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96 in complex with Folinic Acid

1vrq, resolution 2.20Å

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